摘要
本实验旨在建立HPLC同步测定四黄止痢复方中黄芩苷和小檗碱的方法,四黄止痢复方中草药经甲醇提取,以甲醇-0.1%磷酸水为流动相进行梯度洗脱,C18色谱柱分离,278 nm波长检测。结果表明:在0.01～0.12 mg/L浓度呈良好的线性关系,加样回收率分别为(98.058±0.897)%、(98.401±0.882)%;重复性良好,相对标准偏差<1%。因此本法适用于四黄止痢复方中黄芩苷和小檗碱含量测定。
A high performance liquid chromatography(HPLC)method was developed for the simultaneous quantitation of baicalin and berberine in compound Chinese medicine Sihuangzhili.The samples were extracted in methanol,the HPLC separation was achieved on C18 column using mobile phase of methanol:0.1%phosphate acid water.The data collection was set at 278 nm.The method provided good linearity from 0.01 mg/L to 0.12 mg/L.The mean recoveries(n=6)of berberberine and baicalin in samples were(98.058±0.897)%and(98.401±0.882)%,respectively and there was a good repeatability(RSD<1%).It was a simple,rapid and reliable method for the simultaneous determination of baicalin and berberine in compound Chinese medicine Sihuangzhili.
作者
王培
钟文诗
孙嘉一
徐子恒
蒋红
王宏军
WANG Pei;ZHONG Wenshi;SUN Jiayi;XU Ziheng;JIANG Hong;WANG Hongjun(College Animal Husband and Veterinary,Jinzhou Medical University,Jinzhou,Liaoning Province 121001,China)
出处
《中国饲料》
北大核心
2019年第9期84-86,共3页
China Feed
基金
国家级大学生创新创业训练计划项目(201710160000176)
国家自然科学基金(31201951)
辽宁省高等学校优秀人才支持计划(LJQ2013087)
辽宁省自然科学基金(201805500687)
关键词
高效液相色谱
四黄止痢
黄芩苷
小檗碱
high performance liquid chromatography
Sihuangzhili
baicalin
berberine
