摘要
以纳米金修饰电极为固定基质,建立一种基于金属有机框架的电化学生物传感器检测赭曲霉毒素A的方法。将核酸修饰的金属有机框架为信号探针,通过碱基互补识别作用将其捕获到适配体修饰的电极表面,此时可获得明显的电信号。当赭曲霉毒素A与适配体特异性结合后无法成功捕获金属有机框架,从而导致电信号降低。在0.1~5.0 nmol/L范围内,赭曲霉毒素A浓度与电信号呈良好的线性关系,检测限为0.03 nmol/L。该方法可成功用于红酒中赭曲霉毒素A的测定并有望用于食品中多种有害物质的实时检测。
Employing a gold nanoparticle-modified electrode as immobilization substrate,a novel electrochemical biosensor has been developed for the sensitive detection of ochratoxin A based on metal organic framework. Nucleic acid-modified metal organic framework was used as a signal probe,which can be captured onto the surface of aptamer-modified electrode by base pairing and generated obvious electrochemical signal. The specific binding of ochratoxin A with the aptamer hindered the capture of metal organic framework,resulting in the decrease of electrochemical signal. The concentration of ochratoxin A was in good linear relationship with electrochemical signals in the range of 0. 1 ~5. 0 nmol/L,and the detection limit was 0. 03 nmol/L. This method can be successfully applied to the determination of ochratoxin A in red wine. The electrochemical biosensor is easy to operate,low cost,highly sensitive and selective. It has great potential for real-time detection of many harmful substances in food.
作者
王艺伟
刘俏
黄群
安凤平
宋洪波
WANG Yi-wei;LIU Qiao;HUANG Qun;AN Feng-ping;SONG Hong-bo(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 35000)
出处
《分析试验室》
CAS
CSCD
北大核心
2018年第8期880-883,共4页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金项目(21605019)
福建省自然科学基金项目(2015J01083)
福建农林大学高水平大学建设项目(612014042)资助
