摘要
高灵敏的生物传感器在痕量真菌毒素污染的快速检测中备受关注。适配体除具有与抗体类似的高选择性外,还具有可体外合成和易修饰等独特优势,已成为现阶段生物传感器中常用的识别元件。随着指数富集的配体系统进化(SELEX)技术的发展,筛选获得的真菌毒素适配体越来越多,为不同真菌毒素的检测提供了基础条件,而适配体结合现代新型纳米材料扩展了生物传感体系中生物识别与传感的范围。光学和电化学生物传感器技术属于目前极具吸引力的分析方法,介绍了近年来基于适配体的光学和电化学生物传感器检测真菌毒素的构建原理、方法特点和相关应用进展,并提出了面临的挑战与发展趋势,以期为真菌毒素检测相关研究的开展提供参考。
Biosensors with higher sensitivity in rapid detection of trace mycotoxin contamination have attracted world attention.In addition to high selectivity similar to antibodies,aptamers also have unique advantages such as in vitro synthesis and easy modification,and have become commonly used identification elements in biosensors.With the development of the exponentially enriched ligand system evolution(SELEX)technology,more and more mycotoxin aptamers have been screened,providing basic conditions for the detection of different mycotoxins.In addition,the integration of aptamers and novel nanomaterials have extended the scope of biometric recognition and sensing in biosensing systems.In recent years,optical and electrochemical biosensors are quite fascinating detection modes for ultrasensitive screening of mycotoxin.Thus,this review summarized the principles,characteristics and the application progress of these two aptamer-based biosensors,as well as discussed the current problems and future prospect.
作者
关凯仪
黄仁堂
张磊
王淑美
GUAN Kaiyi;HUANG Rentang;ZHANG Lei;WANG Shumei(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006;Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of SATCM Engineering Technology Research Center for Chinese Materia Medica Quality of Guangdong Province,Guangzhou 510006;School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006)
出处
《分析试验室》
EI
CAS
CSCD
北大核心
2022年第2期212-224,共13页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金(81903798)
2020年广东省科技创新战略专项资金(pdjh2020b0302)
国家级外专项目(G20190001631)资助。
