摘要
苯丙酮尿症等代谢病患者因基因缺陷无法正常代谢食物中的芳香族氨基酸(aromatic amino acids,AAA),常规饮食可能会造成永久性生理损伤,低AAA肽是其重要的蛋白质等同物来源。AAA分离分析技术对低AAA肽的制备和检测至关重要。该领域研究者探索了多种高效的吸附、分离材料,从复杂的蛋白质水解液中选择性吸附脱除AAA以制备符合特定氨基酸限量的低AAA肽类食品,或者建立对AAA特异性提取富集的分离分析策略。该文分析了AAA的结构特点与理化性质,总结了近年来基于活性炭、树脂等吸附材料脱除AAA的技术进展,并从样品前处理、手性分离和吸附-传感3个维度综述了二维纳米材料、分子印迹、环糊精、金属有机骨架等材料在AAA分离分析中的应用。通过探讨各类技术的优缺点,为AAA吸附脱除和分离分析技术的进步提供支撑。
Amino acids are important building blocks of proteins in the human body,which are involved in many metabolic pathways.Patients with metabolic diseases such as phenylketonuria,tyrosinemia,and hepatic encephalopathy are genetically defective and cannot metabolize aromatic amino acids(AAA)in food;hence,a regular diet may lead to permanent physiological damage.For this reason,it is necessary to restrict the intake of AAA in their daily diet by limiting natural protein intake,while ensuring normal intake of low protein foods and supplementation with low-AAA protein equivalents.Sources of low-AAA protein equivalents currently rely on free amino acid complex mixtures and low-AAA peptides(also known as high-Fischer-ratio peptides),which have better absorption availability and palatability.AAA separation and analysis techniques are essential for the preparation and detection of low-AAA peptides.Researchers in this field have explored a variety of efficient adsorption materials to selectively remove AAA from complex protein hydrolysates and thus prepare low-AAA peptide foods,or to establish analysis strategies for AAA.Covering more than 70 publications on AAA removal and separation in the last decade from Web of Science Core Collection and China National Knowledge Infrastructure,this review analyzes the structural characteristics and physicochemical properties of AAA,and summarizes the technological progress of AAA removal based on adsorbents such as activated carbon and resin.The applications of two-dimensional nanomaterials,molecular imprinting,cyclodextrins,and metal-organic frameworks in AAA adsorption and analysis from three dimensions,i.e.,sample pretreatment,chiral separation and adsorption sensing,are also reviewed.The mainstream adsorbents for AAA removal,such as activated carbon,still suffer from poor specificity and cause environmental pollution during post-use treatment.Existing AAA separating materials show impressive selective adsorption capability in food samples and chiral mixtures as well as high sensitivi
作者
鲁晨辉
张毅
苏宇杰
王文龙
冯永巍
LU Chenhui;ZHANG Yi;SU Yujie;WANG Wenlong;FENG Yongwei(State Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;Technology Innovation Center of Special Food for State Market Regulation, Wuxi Food Safety Inspection and Test Center, Wuxi 214142, China)
出处
《色谱》
CAS
CSCD
北大核心
2022年第8期686-693,共8页
Chinese Journal of Chromatography
基金
江苏省市场监督管理局科技计划项目(KJ204131)
国家自然科学基金(21876066)
河北省医学科学研究课题计划(20211856)
江南大学基础科研计划(JUSRP21921)
江苏省食品安全与质量控制协同创新中心项目.
关键词
芳香族氨基酸
吸附
脱除
分离
综述
aromatic amino acids
adsorption
removal
separation
review
