摘要
[Objectives] The aim of this work was to compare the inhibitory effects of different water-soluble extracts of ginseng on hydroxyl radical. [Methods]The water-soluble effective parts of ginseng were extracted by orthogonal test,the hydroxyl radical scavenging rate of each extract was determined by salicylic acid method,and the extraction process of the optimum hydroxyl radical inhibitor was optimized using variance analysis and cluster analysis. [Results] A total of 16 samples were prepared. Among them,the sample No. 12 had the highest hydroxyl radical scavenging rate,and the inhibition rate reached 105. 08% at the concentration of 1 g/L. [Conclusions] Ginseng water extract contained hydroxyl radical scavenger,of which the optimum extraction process was as follows: p H of 8,solid to liquid ratio of 1∶ 10,extraction temperature of 4℃,extraction time of 12 h and extraction times of 3.
College of Pharmacy/Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Application, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China; 2. Department of Detection, Huaxin Physical and Chemical Analysis Center Co., Ltd., Dalian 116000, ChinaAbstract [Objectives] The aim of this work was to compare the inhibitory effects of different water-soluble extracts of ginseng on hydroxyl radical.[Methods] The water-soluble effective parts of ginseng were extracted by orthogonal test, the hydroxyl radical scavenging rate of each extract was determined by salicylic acid method, and the extraction process of the optimum hydroxyl radical inhibitor was optimized using variance analysis and cluster analysis.[Results] A total of 16 samples were prepared. Among them, the sample No.12 had the highest hydroxyl radical scavenging rate, and the inhibition rate reached 105.08% at the concentration of 1g/L.[Conclusions] Ginseng water extract contained hydroxyl radical scavenger, of which the optimum extraction process was as follows： pH of 8, solid to liquid ratio of 1∶ 10, extraction temperature of 4℃, extraction time of 12 h and extraction times of 3.
基金
Supported by Open Foundation of Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications,Liaoning University of Traditional Chinese Medicine(ZYZX1709)
Project of Education Department of Liaoning Province(L201606)
China Postdoctoral Science Foundation(2013M530945)
