摘要
目的:比较不同年限人参中水溶性蛋白的含量,以鉴别人参质量的年限差异。方法:选取吉林省抚松县同一产地、不同年限的4批人参,应用Bradford蛋白质定量试剂盒测定水溶性蛋白含量,采用十二烷基硫酸钠-聚丙稀酰胺凝胶电泳(SDS-PAGE)法获得水溶性蛋白的电泳胶片,通过凝胶成像软件将胶片转化成含量-比移值图谱,累积叠加不同年限人参的图谱,比较分析不同条带的含量变化情况。结果:不同年限人参中水溶性蛋白的SDS-PAGE谱带条数无明显差异,主要谱带为9条,但因含量的不同而引起的谱带指纹信息差异较大。结论:人参中水溶性蛋白的含量在不同年限间存在差异,以4年生含量最低,5～7年生没有明显差异。
OBJECTIVE： To compare the content of water-soluble protein in Panax ginseng of different years, and to identify the quality of P ginseng with the different years. METHODS： 4 batches of P ginseng of different year from Fusong county of Changbai mountain area were selected, and the content of water-soluble protein was determined by Bradford quantitative kit; we get the electrophoresis spectrum about water-soluble protein of P ginseng with SDS-PAGE. The electrophoresis spectrum was trans- formed into conccntration-Rf spectrum through the gel imaging software： the spectrums of water-soluble protein in P ginseng from different years were superimposed, and the concentrations of different bands were compared. RESULTS： There was no significant difference in SDS-PAGE bands of water-soluble protein in P ginseng of different years. There were 9 main absorption peak, and the number of absorption peaks are similar, but the different of fingerprint was great among different concentrations. CONCLU- SIONS： The contents of water-soluble protein are different in P ginseng of different years; the content of 4 years is the least, and the content of 5-7 years haven＇t the obvious differences.
出处
《中国药房》
CAS
CSCD
2013年第19期1779-1781,共3页
China Pharmacy
基金
国家科技重大专项课题资助(No.2011ZX09401-305)
国家科技支撑计划资助课题(No.2012BAI29B05)
吉林省医药产业发展专项资金项目(No.YYZX201134)
