摘要
【目的】通过中药美白成分的复配,进而研究复方中药发酵液活性成分及功效性。【方法】以酪氨酸酶抑制率为指标研究最佳复配含量,采用分光光度法测定复方中药发酵液中总酚、多糖、黄酮、蛋白质含量,通过HPLC技术进行氨基酸分析,抗氧化能力通过总还原力和自由基清除试验进行评价,通过酪氨酸酶活性抑制实验进行美白功效分析。【结果】正交实验得出4种中药美白成分中覆盆子对酪氨酸酶抑制效果最好;复方中药发酵液中多酚、多糖、黄酮和蛋白质含量分别为147.80、4.36、1.17、2.22 g/kg;复方中药发酵液具有较高的总还原力、羟自由基清除能力和DPPH自由基清除能力;5%(W/V)复方中药发酵液的酪氨酸酶活性抑制率为82.41%。【结论】复方中药发酵液中含有多种活性成分,具有一定的抗氧化和美白功效。
[Objective] Through complex of whitening ingredients from different Chinese herbs, we studied the active ingredients and effectiveness of compound Chinese herbs fermentation broth. [Methods] The tyrosinase inhibition rate was used as the index, the best compound composition was obtained. The contents of total polyphenols, polysaccharides, flavonoids, and protein in the fermentation broth were measured by UV spectrophotometry, and amino acids compositions were analyzed by HPLC. We evaluated their antioxidant activity in fermentation broth by total reducing power and radical scavenging tests. The whitening efficacy was evaluated by tyrosinase inhibition test. [Results] Rubifructus had the best inhibition of tyrosinase activity by orthogonal designed experiment. The fermentation broth of compound Chinese herbs contained 147.8 g/kg of total polyphenols, 4.360 g/kg of polysaccharides, 1.170 g/kg of flavonoids and 1.220 g/kg of total protein. The fermentation broth of compound Chinese herbs had strong total reducing power, hydroxyl radical scavenging ability and 1,1-diphenyl-2-picrylhydrazyl（DPPH） radical scavenging activity. Tyrosinase inhibition rate of 5%（W/V） compound Chinese herbs fermentation broth reached 82.41%. [Conclusion] The fermentation broth of compound Chinese herbs contained multiple active ingredients, with antioxidant activity and whitening efficacy.
作者
李瑜
顾秋亚
刘咏梅
余晓斌
Yu Li;Qiuya Gu;Yongmei Liu;Xiaobin Yu(Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,Jiangsu Province,China;School of Pharmacy,Taizhou Polytechnic College,Taizhou 225300,Jiangsu Province,China)
出处
《微生物学报》
CAS
CSCD
北大核心
2018年第8期1483-1491,共9页
Acta Microbiologica Sinica
关键词
中药
复配
发酵
活性成分
抗氧化
美白
Chinese herb
complex
fermentation
active ingredients
anti-oxidation
whitening
