摘要
[Objective] The paper was to sequence the rbcL gene of several species belonging to Morus, and to explore the genetic relationship of Morus plants. [Method] Through DNA extraction, PCR amplification, sequencing, splicing and correction, a total of 56 rbcL gene sequences were obtained. [Result] Alignment results showed that there were 1 279 permutation sites in rbcL sequence of Morus plants; variable sites accounted for 6.8% of the total sequence length, and the ratio of transition pairs to transversion pairs （R= si/sv） was 1.2. The rbcL sequence of Broussonetia papyrifera was selected as the outgroup, which was downloaded from GenBank. Genetic analysis results showed that M. alba, M. notabilis, M. rubra and Kuisang （the sample of this study） had distant relationship, and the other Morus plants had relatively close relationship. [Conclusion] The results lay a foundation for selection, identification and classification of Morus plants.
[Objective] The paper was to sequence the rbcL gene of several species belonging to Morus, and to explore the genetic relationship of Morus plants. [Method] Through DNA extraction, PCR amplification, sequencing, splicing and correction, a total of 56 rbcL gene sequences were obtained. [Result] Alignment results showed that there were 1 279 permutation sites in rbcL sequence of Morus plants; variable sites accounted for 6.8% of the total sequence length, and the ratio of transition pairs to transversion pairs （R= si/sv） was 1.2. The rbcL sequence of Broussonetia papyrifera was selected as the outgroup, which was downloaded from GenBank. Genetic analysis results showed that M. alba, M. notabilis, M. rubra and Kuisang （the sample of this study） had distant relationship, and the other Morus plants had relatively close relationship. [Conclusion] The results lay a foundation for selection, identification and classification of Morus plants.
基金
Supported by Basic and Frontier Research Project of Chongqing City(cstc2015jcyj A80010)
Basic Scientific Research Expense Project of Chongqing City(16441)
Agricultural Finance Project of Chongqing Agricultural Committee(15403)
