摘要
目的：观察开口箭皂苷对甲状腺髓样癌TT细胞（MTC-TT）及荷瘤小鼠模型中SUMO特异性蛋白酶2（SENP2）的水平变化,探讨开口箭皂苷治疗甲状腺髓样癌的作用机制。方法：不同浓度的开口箭皂苷作用于MTC-TT细胞,分别给予2、4、8、16、32、64μg/m L开口箭皂苷处理24、48、72 h,采用CCK8法检测各浓度下MTCTT细胞增殖抑制率并计算出半数抑制浓度（IC50）,将实验分为4组,低浓度组（TL组,浓度为0.5 IC50开口箭皂苷）、中浓度组（TM组,浓度为IC50开口箭皂苷）、高浓度组（TH组,浓度为2 IC50开口箭皂苷）和对照组（N组,培养基中无开口箭皂苷）。将药物作用于各浓度组MTC-TT细胞48 h后,流式细胞术检测细胞凋亡;Western blot检测MTC-TT细胞中SENP2的蛋白表达;RT-PCR检测MTC-TT细胞中SENP2的基因表达。结果：开口箭皂苷对MTC-TT细胞增殖有抑制作用（P〈0.05）,且随浓度增加抗增殖作用越强（P〈0.05或P〈0.01）,开口箭皂苷对MTCTT细胞的IC50值为31.54μg/m L;TM组和TH组G0/G1期细胞相对减少（P〈0.05或P〈0.01）,TL、TM、TH组G2/M期细胞增加（P〈0.05或P〈0.01）,细胞阻滞在G2/M期;TT细胞株中SENP2的蛋白和m RNA表达水平均显著下降。结论：开口箭皂苷可以抑制MTC-TT细胞增殖和诱导其凋亡,其作用机制可能与抑制SENP2的表达有关。
Objective：To observe the effect of total saponins of Tupistra chinensis（TST）on expression of SUMO specific protease 2（SENP2）in the medullary thyroid carcinoma TT（MTC-TT）cells and tumor bearing mice,and explore the mechanism of TST in treating MTC. Methods：Treating TT cells with TST at 24,48,72 h and different concentration（2,4,8,16,32,64 μg/m L）respectively,the inhibitory rate of MTC-TT cells was measured by CCK8 and the inhibitory concentration（IC50）was calculated. The experiment was divided into four groups：low concentration group（TL group）with 0.5 IC50 TST,medium concentration group（TM group）with IC50 TST,high concentration group（TH group）with 2 IC50 TST,the control group（N group）without TST. After the cells had been treated with different concentration of TST for 48 h,the cell apoptosis was detected by flow cytometry. RT-PCR and Western blot were used to detect the m RNA and protein expression of SENP2 in MTC-TT cells and tumor tissues. Results：TST had the inhibitory action on the proliferation of MTC-TT cells（P〈0.05）,and the stronger the anti proliferation effect with the increase of concentration（P〈0.05 or P〈0.01）,the IC50 value of TST to MTC-TT cells was 31.54 μg/m L,and cells in TM and TH groups decreased in G0/G1（P〈0.05 or P〈0.01）,cells in TL,TM and TH groups decreased in G2/M（P〈0.05 or P〈0.01）,arrested cells at G2/M phase;and the expression levels of SENP2 and m RNA in TT cell lines were significantly decreased. Conclusion：TST could inhibit MTC-TT cell proliferation and induce apoptosis,which may be related to the inhibition of SENP2 in MTC-TT cells.
作者
夏玉坤
艾望
张莹雯
XIA Yukun;AI Wang;ZHANG Yingwen(Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei, China)
出处
《辽宁中医药大学学报》
CAS
2018年第7期46-49,共4页
Journal of Liaoning University of Traditional Chinese Medicine
基金
湖北省自然科学基金青年项目(2015CFB640)
湖北省自然科学基金项目(2009CHB001)
关键词
开口箭皂苷
SUMO特异性蛋白酶2
甲状腺髓样癌
total saponins of Tupistra ehinensis
SUMO specific
protease 2
medullary thyroidcarcinoma
