摘要
目的分析衢州市HIV确证实验室HIV抗体初筛阳性样本复检和确证实验结果,为艾滋病检测工作提供技术支持。方法对衢州市HIV确证实验室在2014-2016年收到的HIV抗体初筛阳性标本,采用ELISA法和胶体硒法进行复检,两种试剂任一试剂量阳性则用蛋白免疫印迹法(WB)进行确证。结果 2014-2016年衢州市HIV确证实验室对送检的529份HIV抗体初筛阳性样本进行了复检,复检阳性率为79.8%(422/529),对复检阳性标本进行了WB确证,确证HIV-1抗体阳性385份,HIV-1抗体阳性率为91.2%(385/422),HIV抗体阴性22份(5.2%);HIV抗体不确定15份(3.6%),随访后阳转7份,随访后阴性2份,失访6份。385份确证病例中重卡病例26例,剔除重卡后359例,以男性为主,男女性别比例为4.3:1,主要集中在20~40岁人群(占63.5%)。异性性传播是HIV最主要的传播途径(占74.6%),同性性传播也占较大比例(占21.4%)。结论应进一步加强不确定结果的随访,降低失访率,尽早发现HIV感染者,降低HIV新发感染,遏制艾滋病的蔓延。
Objective To analyze re-examination and confirmatory results on HIV antibody screening positive samples in Quzhou city and provide technical support for AIDS prevention and control. Methods ELISA and quick diagnosis method were used to re-examine HIV antibody screening positive samples collected from 2014 to 2016 in Quzhou city,samples with at least one positive results from above 2 methods were subjected to confirmatory test by Western Blot（ WB） analysis. Results A total of 529 HIV antibody screening positive samples were re-examined by Quzhou HIV confirmatory laboratory,the rate of re-examinations positive was 79. 8%（ 422/529）,the positive samples were confirmed by WB. A total of385 HIV-1 antibody positive cases were confirmed,the positive rate of HIV-1 antibody was 91. 2%（ 385/422）,22 samples were HIV antibody negative（ 5. 2%）,15 samples were uncertain（ 3. 6%）,after follow-up,among them 7 were confirmed to be HIV-1 antibody positive cases,2 cases were confirmed to be negative,6 cases were not traced for follow-up successfully.Among 385 confirmed cases,26 were repeated,there were 359 cases after excluding repeated cases,predominant cases were male,the sex ratio of male and female was 4. 3∶ 1,aged ranged from 20 to 40 years old（ accounted for 63. 5%）,heterosexual transmission was the main route of HIV（ 74. 6%）,homosexual transmission also accounted for a large proportion（ 21. 4%）.Conclusion The follow-up of uncertain results should be further strengthened to reduce the rate of missed visits,early detection of HIV infection,lower HIV infection and to curb the spread of AIDS.
作者
陈旭富
吕磊
王炜
杨瑞军
CHEN Xu-fu;LYU Lei;WANG Wei;YANG Rui-jun(Quzhou Municipal Center for Disease Control and Prevention, Quzhou, Zhejiang 324000, Chin)
出处
《中国公共卫生管理》
2018年第3期371-373,共3页
Chinese Journal of Public Health Management
