摘要
目的回顾性分析2014—2015年广西医科大学第一附属医院艾滋病确证实验室艾滋病病毒(HIV)抗体初筛阳性标本的确证实验结果,探讨蛋白印迹试验(WB)带型与HIV感染的关系。方法对送至本实验室的917例HIV抗体筛查阳性标本,经WB确证后阳性及不确定结果进行整理,用SPSS17进行统计分析。结果初筛阳性标本中,确证阳性855例,阳性率93.2%,确证阴性43例,阴性率4.7%,不确定结果19例,占筛查阳性的2.1%。确证阳性标本中,env类带型阳性率最高,其中gp160最高为100.0%,p55阳性率最低,占38.6%;不确定者中,共有8种带型,gp160p24带型出现最多占31.6%;随访的14例不确定者中,env类不确定带型阳转率最高。HIV-1抗体阳性WB带型分布在不同性别间差异无统计学意义,pol基因编码的p31(χ^2=9.48,P=0.02)及gag基因编码的p55(χ^2=12.45,P<0.01)、p17(χ^2=8.57,P=0.03)和p39(χ^2=11.41,P<0.01)蛋白阳性率在不同年龄组人群间差异有统计学意义。结论env类条带对于HIV阳性的确证具有重要意义;不确定带型中env类最常见,阳转率也最高,应谨慎处理,加强随访;WB带型分布的差异可能有助于流行病学追踪和病原学诊断,应及时对实验室检测数据进行整理分析,提高实验室检测效率。
Objective To analyze retrospectively the confirmatory HIV test results of antibody screening positive samples during 2014-2015 from AIDS prevention laboratory of the first affiliated hospital of Guangxi medical university,and to explore the relationship between the band pattern of western blot (WB) and HIV infection.Methods 917 cases of positive and indeterminate results,of HIV screening positive samples were collected and analyzed by SPSS-17.Results Among the screened positive samples,855 cases (93.2%) were confirmed as HIV positive,43 cases (4.7%) were proved negative and 19 cases (2.1%) had uncertain results.Among the HIV infections,the positive rate of env band was highest,with the highest gpl60 (100%),and lowest p55 positive (38.6%).Among the uncertain results,there were eight types of banding patterns,with gp160p24 band (31.6%),the highest.14 cases were followed up among the uncertain results,and most env band were converted to positive.The distribution of WB band patterns was not statistically different between sex groups.The p31 proteins (X^2 = 9.48,P = 0.02) encoded by the pol gene and the p55 (X^2 = 12.45,P<0.01) ,pi7(X^2 = 8.57,P = 0.03) and p39(X^2=11.41,P<0.01) proteins encoded by the gag gene were statistically different among different age groups.Conclusion Env bands are of great significance for identification of HIV infection.Env band is the most common indeterminate band patterns,with the highest positive conversion rate,which needs attention to be paid to and follow up tests be strengthened.The difference of WB band distribution may be conducive to epidemiological tracking and pathogenic diagnosis.The laboratory test data should be analyzed to improve the efficiency of laboratory testing.
作者
谢思燕
陈学杰
XIE Siyan;CHEN Xuejie(The First Affiliated Hospital,Guangxi Medical University,Nanning 530021,China)
出处
《中国艾滋病性病》
CAS
CSCD
北大核心
2018年第12期1189-1191,1196,共4页
Chinese Journal of Aids & STD
