摘要
从甜瓜根际酸性土壤中分离筛选到1株耐铝甜瓜枯萎病拮抗菌,命名为A2。根据表型、生理生化特征和16S r RNA基因序列相似性分析,将其鉴定为Pseudomonas sp.。菌株A2对甜瓜枯萎病病原菌的相对防效为68.3%,且拮抗能力具有遗传稳定性。相比Al Cl3处理,菌株A2对Al2(SO4)3表现出了更好的耐受性,最高可耐受50mmol/L Al3+。在含有A13+的S-LB培养基中,菌株最适生长温度为30℃;培养基初始p H值的降低会加剧A13+对菌株A2的毒害作用。研究结果可为含活性铝的酸性土壤中甜瓜枯萎病的生物防治提供优良菌株资源和理论基础。
An aluminum-tolerant bacteria strain antagonizing fusarium wilt of muskmelon, A2, was isolated from the rhizospheric soils of muskmelon. Based on the phenotypic, physiological, biochemical characteristics and phylogenetic analysis of 16 S r RNA gene sequences, it was identified as Pseudomonas sp.. A2 showed strong antagonistic effect against Fusarium oxysporum and its relative control effect was 68.3 %. A2 showed higher tolerant ability for al2（SO4）3 than al Cl3, and it was tolerant to 50 mmol/L A1^3＋. The optimal temperature for the growth of A2 in medium containing A1^3＋ was 30 ℃, and the decreasing initial pH of the medium aggravated the toxic effect of A1^3＋ on strain A2. This study would provide a new resource and theoretical basis for the biocontrol of fusarium wilt of muskmelon in acid and aluminum soils.
作者
李怡
彭文涛
李勤奋
邓晓
吴东明
谭华东
武春媛
LI Yi;PENG Wentao;LI Qinfen;DENG Xiao;WU Dongming;TAN Huadong;WU Chunyuan(Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China;Experiment Station of Agricultural Environment Scientific Observation in Daazhou, Ministry of Agriculture, Danzhou, Hainan 571737, China)
出处
《热带作物学报》
CSCD
北大核心
2018年第6期1176-1182,共7页
Chinese Journal of Tropical Crops
基金
国家自然科学基金项目(No.41701307)
海南省自然科学基金面上项目(No.20164169)
中央级公益性科研院所基本科研业务费专项(No.1630042017034
No.1630022017009
No.2018hzs1J007)
中国热带农业科学院环境与植物保护研究所结余经费自主选题专项(No.hzsjy2018001)
关键词
耐铝
细菌
甜瓜枯萎病拮抗菌
耐铝能力
耐铝特性
aluminum-tolerance
bacteria
bacterium antagonizing fusarium wilt of muskmelon
Al-tolerant ability
Al-tolerant characteristics
