摘要
采用平板分离纯化不同来源地茄子健株和青枯病株内生细菌,利用细菌脂肪酸鉴定技术进行鉴定。共获得内生细菌18属28种,健株共分离到内生细菌15属23种,含量为5.96×105CFU·g-1。病株分离到内生细菌14属16种,含量为3.191×108CFU·g-,其中青枯雷尔氏菌含量为3.187×108CFU·g-。根部分离到的内生细菌最多,其次为茎部,叶部最少。不同种植地来源的茄子健株内生细菌的种类数量有较大差异,来自晋江的健株分离到13属17种,来自东洋的健株分离到7属9种,而来自福清的健株仅分离到3属4种。来自晋江的植株内生细菌的SIMPSON(D)优势度指数和SHANNON(H1)多样性指数分别为0.9632和3.5725,均高于来自东洋植株(D:0.9444,H1:2.7255)和福清植株(D:0.8333,H1:1.5)。而均匀度则以来自东洋植株最高(0.9708),来自晋江和福清的植株分别为0.9654和0.9464。不同生长环境下不同生长状态的茄子植株内生细菌群落结构与多样性存在较大的差异。该研究对于通过微生物标记植物生长的生态环境具有重要的实验意义,对于科学说明生防机理具有重要作用。
In order to study the community structure and diversity of endophytic bacteria in the eggplant, the healthy and disease infested eggplant plants were collected from three sites in Fujian Province, PR China. The endophytic bacteria were isolated using the culture method and identified by Microorganism Identification System （MIDI system produced by USA）. The results showed that a total of 28 microbial species identified belonging to 18 genera of bacteria were colonized detected in the eggplant, of which, there were 23 microbial species belonging to 15 genera were found inside the healthy plant with a amount of 5.96×10^5 CFU.g^-1, and 16 microbial species belonging to 14 genera were displayer in the diseased infested plant with a amount of 3.191×10^8 CFU.g^-1, especially Ralstonia solanacearum reached to the amount of 3.187× 10^8 CFU.g^-1. The number of endophytic bacteria decreased in the turn of root, stem, leaves. The significant difference of endophytic bacteria was observed among the eggplants collected from different sites, i.e. 17 species belonging to 13 genera from Jinjiang, 9 species belonging to 7 genera from Dongyang and 4 species belonging to 3 genera from Fuqing. The indices of SIMPSON （D） and SHANNON （H1） for the endophytic bacterium community in the plant from Jinjiang county were 0.963 2 and 3.572 5, respectively, higher than those in the plant from Dongyang and Fuqing counties. The evenness of endophytic bacteria was 0.970 8 in the plant from Dongyang county, which was higher than that of 0.965 4 or 0.946 4 in the plant from Jinjiang county or Fuqing county, respectively. The community structure and diversity of endophytic bacteria were significant different in the healthy conditions and the cultivated sites.
出处
《生态环境学报》
CSCD
北大核心
2009年第4期1433-1442,共10页
Ecology and Environmental Sciences
基金
国家自然科学基金项目(30871667)
国家高技术研究与发展计划(863)项目(2006AA10A211)
福建省财政专项-福建省农业科学院科技创新团队建设基金项目(STIF-Y03)
福建省发改委重点项目(闽发改投资[2006]781号)
关键词
茄子植株
内生细菌
群落结构
多样性
eggplant plant
endophytic bacteria
community structure
diversity
