摘要
目的探讨三磷酸腺苷酶家族蛋白2(ATAD2)在不同病变胃组织中的表达及意义。方法收集12例新鲜胃腺癌及相应癌旁相对正常组织标本,利用实时荧光定量PCR方法(RT-qPCR)检测两种组织中ATAD2 mRNA的表达;同时应用免疫组化法检测ATAD2在121例胃腺癌及相应癌旁相对正常组织、121例非瘤变胃组织、29例高低级别上皮内瘤变组织中的表达情况。结果 RT-qPCR方法检测结果表明,与相应癌旁相对正常组织相比,新鲜胃腺癌组织中ATAD2 mRNA表达明显上调,差异具有统计学意义(t=4.664,P<0.01);ATAD2在非瘤变胃组织中均不表达,在高低级别上皮内瘤变中均为低表达,在胃腺癌组织中有23例(23/121)呈高表达。在胃腺癌组织中ATAD2的表达明显高于相应癌旁相对正常组织(z=-12.588,P<0.01);在低级别上皮内瘤变中的表达高于非瘤变胃组织(z=-7.984,P<0.01);在胃腺癌、高级别上皮内瘤变组织中的表达均高于低级别上皮内瘤变组织(z=-2.531、-2.285,P<0.05);在胃腺癌与高级别上皮内瘤变、慢性萎缩性胃炎与慢性浅表性胃炎中的表达差异均不具有统计学意义(P>0.05)。ATAD2在胃腺癌组织中的表达与肿瘤大小、分化程度相关(χ~2=5.506、8.412,P<0.05),而与病人年龄、性别、淋巴结转移、浸润深度、TNM分期无关(P>0.05)。结论 ATAD2参与了胃腺癌的发生发展过程;ATAD2表达检测对临床判定某些胃黏膜病变性质有重要的参考价值。
Objective To examine the expression of ATPase family AAA domain-containing protein 2（ATAD2）in different gastric lesions and its significance. Methods A total of 12 fresh gastric adenocarcinoma specimens and the adjacent normal tissue specimens were collected,and the expression of ATAD2 mRNA was measured using quantitative real-time PCR（RTqPCR）.ATAD2 protein expression in 121 gastric adenocarcinoma and the adjacent normal tissues,121 non-neoplastic gastric tissues,and 29 high-grade and low-grade intraepithelial neoplasia tissues were determined by immunohistochemistry（IHC）. Results The RT-qPCR results showed that ATAD2 mRNA expression was significantly upregulated in fresh gastric adenocarcinoma tissues than in the adjacent normal tissues（t=4.664,P〈0.01）.IHC staining revealed that ATAD2 was not expressed in non-neoplastic gastric tissues,lowly expressed in high-grade and low-grade intraepithelial neoplasia tissues,and highly expressed in 23（23/121）gastric adenocarcinoma tissues.In addition,ATAD2 protein expression was significantly higher in gastric adenocarcinoma tissues than in the adjacent normal tissues（z=-12.588,P〈0.01）,and higher in low-grade intraepithelial neoplasia tissues than in non-neoplastic tissues（z=-7.984,P〈0.01）.We also found higher ATAD2 protein expression in gastric adenocarcinoma and high-grade intraepithelial neoplasia tissues than in low-grade intraepithelial neoplasia tissues（z=-2.531,-2.285,respectively,both P〈0.05）.However,there was no significant difference in the level of ATAD2 expression between gastric adenocarcinoma and high-grade intraepithelial neoplasia and between chronic atrophic gastritis and chronic superficial gastritis（both P〉0.05）.Furthermore,ATAD2 expression in gastric adenocarcinoma tissues was correlated with tumor size and degree of differentiation（χ^2=5.506,8.412,respectively,both P〈0.05）,but not with age,sex,lymph node metastasis,depth of infiltration,and TNM stage（P〉0.05）. Conclusion ATAD2 is involved in the d
作者
季惠惠
周璇
徐进
李海艳
项锋钢
JI Huihui,ZHOU Xuan,XU Jin,LI Haiyan,XIANG Fenggang(Department of Pathology,Qingdao University Basic Medicine College,Qingdao 266021, Chin)
出处
《精准医学杂志》
2018年第2期162-165,共4页
Journal of Precision Medicine
基金
国家自然科学基金资助项目(81502388)
关键词
三磷酸腺苷酶家族蛋白2
胃肿瘤
病理学
临床
免疫组织化学
ATPase family AAA domain-containing protein 2
Stomach neoplasms
Pathology
clinical
Immunohistochemistry
