摘要
目的:为实现现场快速检测牛肉及其制品中牛DNA成分,建立基于荧光RPA技术的快速检测方法。方法:在细胞色素C氧化酶亚型Ⅰ中的黄牛、水牛、牦牛特异性DNA序列上设计RPA引物以及exo探针,对引物对进行组合筛选,结合核酸快速提取方法,建立荧光RPA快速检测方法,并对其特异性、灵敏度测试,通过方法比对实验验证其检测准确性。结果:所建立的检测方法具有很好的特异性,检测灵敏度低至1%(w/w),并且与标准方法检测结果一致。结论:研究成功建立了牛肉真伪鉴别荧光RPA现场快速检测方法。
Purpose： Construct beef authentication on-site rapid detection method based on fluorescent RPA technology, in order to realize rapid detection of bovine DNA components in beef and its products. Methods： Design RPA primer pairs and exo probe according to COI gene sequences specific for cow, buffalo and yak. Construct bovine authentication on-site rapid detection method through primer pairs screening, specificity and sensitivity test. Results： This method has good specificity, and the sensitivity can be reached 1%（w/w）. The testing results were the same with standard methods. Conclusions： This study successfully construct beef authentication on-site rapid detection method based on fluorescent RPA technology.
作者
林霖
冯荣虎
王坤
余灏
张世伟
赖心田
杨国武
LIN Lin;FENG Rong-hu;WANG Kun;YU Hao;ZHANG Shi-wei;LAI Xin-tian;YANG Guo-wu(Shenzhen Academy of Metrology ＆ Quality Inspection, Shenzhen 518131)
出处
《食品科技》
CAS
北大核心
2018年第6期322-326,共5页
Food Science and Technology
基金
广东省质量技术监督局科技项目(2016PZ11)
关键词
牛肉
真伪鉴别
RPA
现场快检
beef
adulteration
RPA
on-site rapid detection
