摘要
目的:建立气相色谱法测定牛黄清心丸(局方)中龙脑和异龙脑含量的方法。方法:采用HP-INNOWAX毛细管柱(30 m×0.25 mm,0.25μm),柱温110℃,进样口温度200℃;FID检测器温度230℃;载气为氮气;载气流速1.8 ml·min^(-1),分流比10∶1;进样量:1μl。结果:龙脑和异龙脑分别在0.01~5.09μg·ml^(-1)(r=0.999 5)和0.01~5.03μg·ml^(-1)(r=0.999 1)范围内线性关系良好,平均回收率分别为99.34%和99.24%,RSD分别为0.59%和0.62%(n=6)。结论:所建方法准确、灵敏、简便,可用于牛黄清心丸(局方)的质量控制。
Objective： To establish a GC method for the determination of borneol and isoborneol in Niuhuang Qingxin pills（ prescription of the bureau）. Methods： A capillary column HP-INNOWAX（ 30 m×0. 25 mm,0. 25 μm） was used and the column temperature was kept at 110 ℃. The temperature of the inlet and the FID was 200 ℃ and 230 ℃,respectively. The carrier gas was N2 and the flow rate of the carrier gas was 1. 8 ml·min-1. The split ratio was 10 ∶1,and the injection volume was 1 μl. Results： The linear response range of borneol and isoborneol was 0. 01-5. 09 μg·ml-1（ r=0. 999 5） and 0. 01-5. 03 μg·ml-1（ r = 0. 999 1）,respectively. And the average recovery was 99. 34% and 99. 24% with the RSD of 0. 59% and 0. 62%,respectively（ n = 6）. Conclusion： The proposed method is accurate,sensitive and simple,and can be used for the quality control of Niuhuang Qingxin pills（ prescription of the bureau）.
作者
聂黎行
戴忠
马双成
Nie Lixing;Dai Zhong;Ma Shuangcheng(National Institutes for Food and Drug Control,Beijing 100050, China)
出处
《中国药师》
CAS
2018年第6期981-983,共3页
China Pharmacist
基金
国家自然科学基金项目(编号:81303194)
国家中医药管理局中药标准化建设项目(编号:ZYBZH-C-JS-32)
中国食品药品检定研究院学科带头人培养基金项目(编号:2017X1)
关键词
牛黄清心丸(局方)
龙脑
异龙脑
含量测定
气相色谱法
Niuhuang Qingxin pills（prescription of the bureau）
Borneol
Iso-borneol
Determination
GC
