摘要
目的探索KIF2C在肝细胞癌(HCC)组织中的表达及其临床意义。方法收集唐山市人民医院2009-2013年手术切除的肝细胞癌肿瘤组织及匹配的癌旁组织各104例,采用免疫组化的方法检测肿瘤及癌旁组织中KIF2C的表达水平,并结合Oncomine数据库中的数据对KIF2C在肝癌中的表达情况进行大样本验证。此外,用Kaplan-Meier法分析KIF2C表达高低与患者总生存率(OS)及无瘤生存率(DFS)的关系。结果 HCC组织中KIF2C高表达者占68.27%(71/104),明显高于癌旁组织的20.19%(21/104),且差异有统计学意义(P<0.05);Oncomine数据库中225例HCC及220例癌旁组织标本的KIF2C表达水平验证结果提示,肝癌组织中KIF2C的表达水平明显高于癌旁组织,且差异有统计学意义(P<0.05);KIF2C的表达与患者年龄、肿瘤的大小及肿瘤分化程度以及TNM分期有关(P<0.05);Kaplan-Meier生存分析显示,KIF2C低表达组患者的总生存率和无瘤生存率明显优于KIF2C高表达组。结论 KIF2C在HCC的发生发展过程中可能发挥着重要的作用,KIF2C有望成为HCC治疗的一个潜在靶点,且该基因可以作为HCC患者预后的一个有效的预测因子。
Objective To investigate the expression of KIF2 C in hepatocellular carcinoma（HCC） and its clinical significance. Methods A total of 104 cases of hepatocellular carcinoma tissues and matched para-cancer tissues were collected from Tangshan People＇s Hospital from 2009 to 2013. The expression of KIF2 C in HCC tissues and adjacent normal tissues was detected by immunohistochemistry, and the prognosis of patients was verified with the data in the Oncomine database. Furthermore, Kaplan-Meier analysis was used to evaluate overall survival（OS） and disease-free survival（DFS） of HCC patients. Results The high KIF2 C expression of HCC tissue accounted for 68.27%（71/104）,which was significantly higher than 20.19%（21/104） of adjacent tissues, and the difference was statistically significant（P〈0.05）. The verification results of KIF2 C expression in 225 cases of HCC and 220 paraneoplastic tissues in the Oncomine database suggested that the expression level of KIF2 C in HCC tissues was significantly higher than that in the adjacent tissues（P〈0.05）. The expression of KIF2 C was associated with the age of the patients, the size of the tumor,the degree of tumor differentiation, and the TNM stage（P〈0.05）. Kaplan-Meier analysis revealed that the overall survival rate of patients with low KIF2 C expression was significantly better than that of individuals with high expression.Conclusion KIF2 C may play an important role in the development and progression of HCC. KIF2 C is expected to be a potential target for HCC therapy and this gene may serve as a valid predictor of prognosis in patients with HCC.
作者
赵娟
谷雪
刘建生
ZHAO Juan;GU Xue;LIU Jian-sheng.(Fifth Department of Chemoradiotherapy, Tangshan People＇s Hospital, Tangshan 063000, Hebei, CHIN)
出处
《海南医学》
CAS
2018年第11期1493-1496,共4页
Hainan Medical Journal
关键词
肝细胞癌
KIF2C
表达
临床意义
Hepatocellular carcinoma
KIF2C
Expression
Clinical significance
