摘要
目的探讨微小RNA-30c(miR-30c)在肾癌组织中的表达及其临床病理特征和预后关系。方法收集本院2012年1月至2015年12月经手术切除的95例肾癌组织和82例癌旁组织标本,采用实时定量PCR(QPCR)检测以上组织中miR-30c水平,分析miR-30c表达与肾癌临床病理参数(性别、年龄、TNM分期、淋巴结转移、远处转移和T分期)及预后的关系,采用Cox回归模型进行多因素分析。结果QPCR检测发现95例肾癌组织中miR-30c的表达水平为0.561±0.378,低于82例癌旁组织的1.220±0.820(P<0.05)。肾癌组织中miR-30表达与性别、远处转移和年龄均无关(P>0.05),而与TNM分期、淋巴结转移和T分期均有关(P<0.05),其中Ⅲ~Ⅳ期、淋巴结转移及T_3~T_4期的miR-30c相对表达量分别为0.422±0.219、0.442±0.299和0.408±0.194,均低于Ⅰ~Ⅱ期、无淋巴结转移及T_1~T_2期的0.671±0.439、0.619±0.400和0.654±0.431(P<0.05);Ⅲ~Ⅳ期、淋巴结转移及T_3~T_4期的miR-30c高表达率分别为21.43%(9/42)、16.13%(5/31)和19.44%(7/36),均低于Ⅰ~Ⅱ期、无淋巴结转移及T_1~T_2期的41.51%(22/53)、16.13%(26/64)和40.68%(24/59),差异均有统计学意义(P<0.05)。miR-30c低表达组的中位总生存期为37.0个月,短于高表达组(>60.0个月),差异有统计学意义(P<0.05)。Cox回归模型分析发现,TNM分期、淋巴结转移、远处转移、T分期及miR-30c表达均是影响预后的独立因素。结论肾癌组织中miR-30c低表达,miR-30c参与肾癌的发生发展且与预后有关,其中miR-30c低表达者的预后较差,是影响预后的独立因素。
Objective To investigate the expression of microRNA-30c（miR-30c）in renal cell carcinoma and its relationship with clinicopathological features and prognosis.Methods Ninety-five cases of surgical removed renal cell carcinoma tissues and 82cases of paracancerous tissues from January 2012 to December 2015 were collected.Real-time quantitative PCR（QPCR）was used to detect the level of miR-30c in the above tissues.The relationship between miR-30c expression and clinicopathological parameters（gender,age,TNM stage,lymph node metastasis,distant metastasis and T staging）and prognosis of renal cell carcinoma were analyzed.Cox regression model was used for multivariate analysis.Results QPCR detection showed that the expression level of miR-30c in 95cases of renal cell carcinoma was 0.561±0.378,lower than 1.220±0.820 in 82 cases of adjacent tissues（P〈0.001）.The expression of miR-30 in renal cell carcinoma was not related to sex,age and distant metastasis（P〉0.05）,but related to TNM stage,lymph node metastasis and T stage（P〈0.05）.The relative expressions of miR-30c inⅢ-Ⅳstage,lymph node metastasis and T3-T4stage were 0.422±0.219,0.442±0.299 and 0.408±0.194,lower than 0.671±0.439,0.619±0.400 and 0.654±0.431 inⅠ-Ⅱstage,no lymph node metastasis and T1-T2stage（P〈0.05）.The high expression rates of miR-30c inⅢ-Ⅳstage,lymph node metastasis and T3-T4stage were 21.43%（9/42）,16.13%（5/31）and 19.44%（7/36）,lower than 41.51%（22/53）,16.13%（26/64）and 40.68%（24/59）ofⅠ-Ⅱstage,no lymph node metastasis and T1-T2stage（P〈0.05）.The median overall survival of miR-30c expression in the low level group was 37.0 months,which was significantly shorter than that in the high level group（〉60.0months）,and the difference was statistically significant（P〈0.05）.Cox regression analysis showed that TNM stage,lymph node metastasis,distant metastasis,T stage and miR-30c expression were independent prognostic factors of renal cell carcinoma.Conclusion The low expressi
作者
蔡冰冰
左东波
CAI Bingbing;ZUO Dongbo(Department of Nephropathy,Suizhou Hospital Affiliated to Hubei Medical College,Suizhou 3222805,Chin)
出处
《临床肿瘤学杂志》
CAS
北大核心
2018年第5期429-434,共6页
Chinese Clinical Oncology
关键词
肾癌
微小RNA-30c
临床病理
预后
Renal cell carcinoma
MicroRNA-30c
Clinicopathology
Prognosis
