摘要
目的探讨微小RNA-1468(miR-1468)在胃癌组织中的表达并分析其临床意义。方法收集2013年6月至2015年1月于本院手术切除的胃癌组织123例和对应癌旁组织78例,采用实时定量PCR(QPCR)法检测以上组织中的miR-1468水平并比较其在癌组织和癌旁组织中的表达差异,采用受试者工作特征曲线(ROC)分析组织miR-1468水平诊断胃癌的效能,分析癌组织miR-1468水平与胃癌临床病理特征(性别、年龄、肿瘤大小、Lauren分型、浸润深度、淋巴结转移、组织分化和TNM分期)的关系,采用Kaplan-Meier法进行生存分析并比较胃癌患者中不同miR-1468水平的预后情况。结果胃癌组织的miR-1468水平为2.524±1.282,高于癌旁组织的1.122±0.850(P<0.05);ROC分析显示组织miR-1468水平诊断胃癌的曲线下面积为0.844(95%CI:0.787~0.900),当以约登指数最大的1.199为诊断界值时,其灵敏度和特异度分别为87.0%和74.4%。胃癌组织miR-1468水平与性别、年龄、Lauren分型和组织分化无关(P>0.05),而与肿瘤大小、浸润深度、淋巴结转移和TNM分期有关(P<0.05);51例Ⅲ期胃癌患者中,miR-1468低表达者的中位无病生存期为40.0个月,优于高表达者的29.0个月(P<0.05),而miR-1468低表达者的中位生存期为56.0个月,与高表达组45.0个月的差异无统计学意义(P>0.05)。结论胃癌组织中miR-1468高表达且与肿瘤大小、浸润深度、淋巴结转移和TNM分期有关,高表者的预后较差,在胃癌的诊治和预后预测中有一定价值。
Objective To investigate the expression of microRNA-1468(miR-1468) in gastric cancer and analyze its clinical significance. Methods One hundred and twenty-three gastric cancer tissues and 78 corresponding paracancerous tissues were collected from our hospital from June 2013 to January 2015. The levels of miR-1468 in the above tissues were detected by real-time quantitative PCR(QPCR) and their distribution differences in cancer tissues and paracancerous tissues were compared. The diagnostic efficacy of miR-1468 in gastric cancer tissues was analyzed by receiver operating characteristic curve(ROC). The relationship between miR-1468 level and clinicopathological characteristics of gastric cancer(gender, age, size of tumors, Lauren classification, depth of invasion, lymph node metastasis, tissue differentiation and TNM stage) was analyzed. Kaplan-Meier survival analysis was used to compare the prognosis of different levels of miR-1468 expression in gastric cancer patients. Results The level of miR-1468 in gastric cancer tissues was 2.524±1.282, higher than 1.122±0.850 in adjacent tissues(P<0.05). ROC analysis showed that the area under the curve of miR-1468 in gastric cancer tissues was 0.844(95% CI: 0.787-0.900), and when 1.199 with the largest Yoden index as the diagnostic threshold, the sensitivity and specificity were 87.0% and 74.4%. In 51 gastric cancer patients with stage Ⅲ, the median disease-free survival in the low expression group was 40.0 months, higher than 29.0 months in the high expression group(P<0.05). The median overall survival in the low expression group was 56.0 months, similar to 45.0 months in the high expression group(P>0.05). Conclusion The high expression of miR-1468 in gastric cancer is related to tumor size, depth of invasion, lymph node metastasis and TNM stage. The prognosis of high expression is poor, and it has a certain value in the diagnosis, treatment and prognosis prediction of gastric cancer.
作者
郭东栋
李晓艳
张福林
GUO Dongdong;LI Xiaoyan;ZHANG Fulin(Department of Oncology,Yan'an People's Hospital,Yan'an 716000,China)
出处
《临床肿瘤学杂志》
CAS
北大核心
2019年第5期396-400,共5页
Chinese Clinical Oncology
