摘要
对淋病奈瑟菌WHO-A株进行转录组测序以获取其非编码RNA(sRNA)的信息,通过梯度剂量诱导淋病奈瑟菌头孢曲松耐药株,生物信息学方法筛选并荧光定量PCR验证以研究头孢曲松耐药调控相关sRNA。结果表明:经诱导WHO-A株产生MIC为1μg·mL-1的耐药变异株,野生型WHO-A株存在1 051条sRNA,其中80条与penA、porB、mtrR等8个耐药相关基因间存在互补关系,检测发现10条表达水平随耐药性增高而降低的sRNA,说明部分sRNA参与淋病奈瑟菌头孢曲松耐药性调控。
The Neisseria gonorrhoeae WHO-A strain was transcriptome sequenced to obtain the information of its noncoding RNA（sRNA）,then the strain was induced with gradient dose of ceftriaxone to get resistant strains.A combination of bioinformatic methods and Real-time Quantitative PCR were used to screen and verify the ceftriaxone resistance related sRNAs.The results showed that the mutant resistant gonococcal strains（MIC=1μg·ml^-1）were obtained by drug induction.The wide-type WHO-A strain contained 1 051 sRNAs,of which 80 sRNAs were complementary to8 resistance-related genes,e.g.,penA,porB,mtrR,etc.The expression levels of 10 sRNAs were decreased with the increasing of ceftriaxone resistance.These results indicated that some sRNAs were involved in the ceftriaxone resistance regulation in N.gonorrhoeae.
作者
单彩龙
冯欢欢
李金月
徐向阳
项青青
李端阳
傅夏燕
王佳欣
李国才
SHAN Cailong;FENG Huanhuan;LI Jinyue;XU Xiangyang;XIANG Qingqing;LI Duanyang;FU Xiayan;WANG Jiaxin;LI Guocai(Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses / Jiangsu Key Laboratory of Noncoding RNA/ College of Medicine, Yangzhou University, Yangzhou 225001, China)
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2018年第1期6-10,共5页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家自然科学基金资助项目(81471906)
扬州大学科技创新团队培养对象项目(2016-09)
扬州大学创新创业训练计划项目(2016-17)
