摘要
采用纸片琼脂扩散法检测扬州地区淋病奈瑟菌临床分离株对常用抗生素的耐药性,并研究耐药基因的定位和转移机制。对耐药菌株YZ1008分别提取染色体DNA和质粒DNA,用PCR技术检测氨苄青霉素抗性基因的定位,含抗性基因的质粒DNA转化大肠埃希菌敏感株,观察大肠埃希菌耐药性的变化。结果表明:淋病奈瑟菌扬州分离株对受试的11种抗生素具有严重的耐药性,氨苄青霉素耐药基因在染色体DNA和质粒DNA上都存在,质粒耐药基因的转移可使大肠埃希菌DH5α获得氨苄青霉素抗性。这为淋病奈瑟菌耐药性防控研究积累有益数据。
Paper disc agar diffusion method was used to investigate the antibiotic resistance of some Neisseria gonorrhoeae clinical isolates in Yangzhou area.Following extraction of the chromosomal DNA and the plasmid DNA from an ampicillin resistant strain,PCR was used to detect the location of the ampicillin resistant gene.The plasmid DNA was transformed to the susceptible E.coli strain DH5αto observe the transfer of the ampicillin resistant gene.The results showed that most N.gonorrhoeae isolates in Yangzhou produced resistances to all or most of 11 kinds of the detected antibiotics.The penicillin resistant gene ampicillin existed on either the chromosomal DNA or the plasmid DNA.The transfer of the plasmid DNA made the susceptible E.coli strain DH5αobtain the ampicillin resistance.This work provided the prevention and control of gonococcal drug resistance with useful data.
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2015年第2期10-13,共4页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家级和江苏省重点大学生创新创业计划项目(201411117029Z)
国家自然科学基金资助项目(81471906)
江苏省高校自然科学基金资助项目(14KJB310025)
关键词
淋病奈瑟菌
耐药基因
质粒
转化
染色体
Nesseria gonorrhoeae
drug-resistance gene
plasmid
transformation
chromosome
