摘要
目的:建立左归饮质量标准。方法:采用薄层色谱法对左归饮中的枸杞子进行定性鉴别;采用高效液相色谱法测定左归饮中毛蕊花糖苷、马钱苷以及甘草苷的含量。结果:枸杞子薄层色谱斑点清晰,分离效果良好,阴性样品无干扰;毛蕊花糖苷、马钱苷和甘草苷分别在0.2~20.0μg(R^2=0.999 6)、0.2~20.0μg(R^2=0.999 9)、0.2~20.0μg(R^2=0.999 5)范围内线性关系良好,平均加样回收率分别为100.91%、98.58%和100.06%,RSD分别为2.05%,1.34%和2.01%。结论:所建立的定性定量方法科学、简单、快速、准确度高,可用于左归饮的质量控制。
Objective： To establishthe quality standard for Zuogui yin. Methods： Thin layer chromatography(TLC） was used for qualitative identification of Lycium chinense. High performance liquid chromatography(HPLC） was used to determine the contents of acteoside,logamin and liquirtin. Results： Lycium chinense could be indentified qualitatively by the TLC with chear spots and without interference in negative control. It was shown that the acteoside,logamin and liquirtin had a good linear relationship in the measurement range of 0. 2-20. 0 μg(R-2= 0. 999 6）,0. 2-20. 0 μg(R-2= 0. 999 9）,0. 2-20. 0 μg(R-2= 0. 999 5）. The average recoveries were 100. 91%,98. 58% and 100. 06%,RSD were 2. 05%,1. 34% and 2. 01% respectively. Conclusion： The established qualitative and quantitative methods are scientific,simple,efficient,accurate,which can be a standard for the quality control of Zuoguiyin.
作者
洪世忠
周贞贞
陈卫东
吴德玲
HONG Shizhong;ZHOU Zhenzhen;CHEN Weidong;WU Deling(Anhui University of Chinese Medicine, Hefei Anhui China 230012;China Pharmaceutical University, Jiangsu Nanjing China 210009)
出处
《中医学报》
CAS
2018年第5期830-834,共5页
Acta Chinese Medicine
基金
安徽省教育厅自然科学基金(KJ2017A297)
关键词
左归饮
枸杞子
毛蕊花糖苷
马钱苷
甘草苷
质量控制
高效液相色谱法
薄层色谱法
Zuoguiyin
Wolfberry fruit
verbascoside
loganin
glycyrrhizin
quality control
high performance liquid chromatography
thin-layer chromatography
