摘要
目的:对甘草多糖中甘草酸和甘草次酸采用RP-HPLC法同时测定。方法:用十八烷基硅烷键合硅胶(4.6mm×150 mm;2.5μm)为填充剂,以流动相A:甲醇(0.2 m o l/L)-醋酸铵-冰醋酸(60∶40∶1)和流动相B:甲醇(0.2m o l/L)-醋酸铵-冰醋酸(90∶10∶1)梯度洗脱,柱温为45oC,流速为0.8 m l/m in,检测波长为254 nm。结果:甘草酸和甘草次酸的最低检出浓度分别为0.8和0.7μg/m l,供试品溶液至少在8 h内稳定。甘草酸和甘草次酸重复性试验RSD为1.2%和1.5%(n=6)。结论:该方法简单、准确,可用于甘草多糖中甘草酸和甘草次酸的同时测定。
Object： A RP-HPLC method was developed to determine the content of glycyrrhizic acid and glycyrrhetinic acid from Liquorices Saccharides. Methods： A C18 Column （4.6 mm×250 mm L × ID, 5μm） with gradient elution was used. Mobile phase A consisted of methanol-0.2 mol/L ammonium sulfate-glacial acetic acid （60 ： 40 ： 1） and mobile phase B consisted of methanol ammonium sulfate glacial acetic acid（90 ： 10 ： 1） at a flow rate 0.8 ml/min. The detection wavelength was set at 254 nm. Results： The minimum detection limits of glycyrrhizic acid and glycyrrhetinic acid were 16μg and 14μg with the RSD of 1. 2% and 1.5%, respectively. The test solution was found to be stable for at least 8 hours. Conclusions： The method was simple and accurate. It can be used for the determination of glycyrrhizic acid and glycyrrhctinic acid from liquorices saccharides.
出处
《天津药学》
2005年第5期6-8,共3页
Tianjin Pharmacy
