摘要
目的:研究眼络通颗粒剂的质量控制方法。方法:采用硅胶 G 薄层色谱法对颗粒剂中2种主要成分川芎和三棱进行定性鉴别;用 RP-HPLC 法对葛根素进行含量测定,采用 Kromasil 100-5 C_(18)(4.6mm×250mm,5μm)为色谱柱,甲醇-水(30∶70)作流动相,检测波长为250nm。结果:葛根素进样量在0.06～0.65μg的范围内与峰面积呈良好的线性关系,r=0.9999,日内、日间精密度 RSD 分别为0.85%和0.48%(n=5),高、中、低3个浓度平均加样回收率(n=3)分别为101.2%,101.1%,101.4%,RSD 分别为1.1%,0.94%,0.41%。结论:本法操作简便,重复性好,结果准确可靠。
Objective： To establish a quality control method for Yanluotong granules. Methods： The two main ingredients Rhizoma Chuanxiong and Rhizoma sparganii were identified by silical Gel thin layer chromatography and the puerarin was determined by RP - HPLC with a Kromasil 100 - 5 C18 （4. 6 mm × 250 mm,5μm） column. The mobile phase was methanol -water（ 30： 70）. The flow rate was 0.5 mL · min^-1. The detection wavelength was 250 nm. Results： The calibration curve was linear in the range of 0. 06 - 0. 65μg for puerarin with r = 0. 9999. Intra-day and inter - day precisions were 0.85% and 0. 48% （ n = 5 ）. The average recoveries of high, middle and low three concentration from sample were 101.2% ,101. 1% ,101.4% with RSD = 1. 1% ,0.94% ,0. 41% （n =3）respeotinely. Conclusion： Both methods are simple, reproducible, accurate and reliable. It may be used for the quality control of this granules.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2005年第8期912-915,共4页
Chinese Journal of Pharmaceutical Analysis
基金
浙江省嘉兴市科技局项目资助课题(20021063)
