摘要
目的:探讨桦褐孔菌皂苷清除肝细胞中脂肪堆积的能力,阐明桦褐孔菌皂苷对非酒精性脂肪肝病(NAFLD)的预防效果。方法:MTT法选取桦褐孔菌皂苷对HepG2细胞的安全浓度,将不同浓度(60.000、6.000、0.600、0.060和0.006g·L^(-1))桦褐孔菌皂苷作用于HepG2细胞作为给药组,以不给药无细胞的空白孔为对照组,以不给药的HepG2细胞为模型组。MTT法检测各组HepG2细胞存活率,检测各组HepG2细胞中甘油三酯(TG)的清除率,油红O染色检测各组HepG2细胞形态表现。结果:提取桦褐孔菌皂苷最佳条件为1∶15的料液比、温度为30℃、提取时间为30min、超声功率为50%。与对照组比较,0.060g·L^(-1)桦褐孔菌皂苷组细胞存活率降低,TG清除率升高(P<0.05),细胞中脂质相互融合的现象明显减轻;0.600g·L^(-1)桦褐孔菌皂苷组细胞存活率降低,TG清除率升高(P<0.01),细胞中脂滴数量明显减少。结论:最佳提取条件下桦褐孔菌皂苷提取率明显升高且具有良好的体外降脂活性,对NAFLD有较好的预防效果。
Objective：To investigate the ability of Phaeoporus obliquus saponins in scavenging the fat accumulation of liver cells,and to clarify its effect on the prevention of non-alcoholic fatty liver disease（NAFLD）.Methods：The safe concentrations of Phaeoporus obliquus in the HepG2 cells was selected by MTT method.The cells treated with 60.000,6.000,0.600,0.060,and 0.006 g·L-1 Phaeoporus obliquus saponins were regarded as treatment groups;the blank well without drugs and cells was regarded as control group,and the HepG2 cells without drugs were regarded as model group.The survival rates of HepG2 cells in various groups were detected by MTT method;the clearance rates of triglyceride（TG）in the HepG2 cells in various groups were detected;the morphology of HepG2 cells was detected by oil red O staining.Results：The optimum extraction condition of Phaeoporus obliquus saponins were as followed：the liquid ratio was 1∶15,the extraction temperature was 30℃,the extraction time was 30 min,and the ultrasonic power was 50%.Compared with control group,the survival rate of HepG2 cells in 0.060 g·L-1 Phaeoporus obliquus saponins group was decreased and the clearance rate of TG was increased（P〈0.05）,and the lipid interfusion in cells was significantly reduced.Compared with control group,the survival rate of HepG2 cells in 0.600 g·L-1 Phaeoporus obliquus saponins group was decreased and the clearance rate of TG was increased（P〈0.01）,and the number of lipid drops was significantly reduced.Conclusion：The extraction rate of Phaeoporus obliquus saponins is obviously improved under the optimum extraction conditions and it has good in vitrolipid-lowering activity,and it has better preventive effect on NAFLD.
作者
张蕾
张琨
王德利
任小红
崔娜
刘洋
赵庭勋
ZHANG Lei;ZHANG Kun;WANG Deli;REN Xiaohong;CUI Na;LIU Yang;ZHAO Tingxun(Department of Pharmaceutical Engineering,College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang 157011,China;Research Center,Second Hospital,Jilin University,Changchun 130041,China;Domonstration Center for Biological Experiments,School of Life Sciences,Jilin University Changchun 130012,China;Center of Pharmaceutical Preparations,Chinese Academy of Sciences,Shanghai Institute of Materia Medica,Shanghai 201210,China)
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2018年第3期526-531,696,共7页
Journal of Jilin University：Medicine Edition
基金
黑龙江省科技厅重点创新预研项目资助课题(SY2014009)
