摘要
目的观察在常规剂量下使用何首乌不同炮制品的水煎液、醇提液、配方颗粒对大鼠肾脏的损伤情况及对肾细胞凋亡及相关蛋白表达的影响。方法 SD大鼠雌雄各半,分别按照体重随机分为7组,即正常对照组(CG)、生首乌水提组(SW组)、制首乌水提组(ZW组)、生首乌醇提组(SA组)、制首乌醇提组(ZA组)、生首乌配方颗粒组(SK组)、制首乌配方颗粒组(ZK组)。其中正常组给予蒸馏水灌胃,其余各组给予相应药液灌胃[生药6g/(kg·d)],连续给药30 d。实验结束时,检测血清尿素氮(BUN)、肌酐(Crea)、尿酸(UA)、β2-微球蛋白(β2-MG)的含量变化,并观察肾脏病理组织形态变化、细胞凋亡情况。结果血清尿素氮含量各给药组均较正常对照组显著降低(P<0.05,P<0.01);肌酐、尿酸、β2-微球蛋白含量各给药组均较正常对照组有升高趋势,制首乌醇提药液组β2-微球蛋白含量显著升高(P<0.05)。TUNEL染色结果表明,各给药组平均光密度值相对于正常对照组均有显著升高(P<0.01),生首乌醇提药液组BAX蛋白表达水平升高最明显(P<0.05,P<0.01)。结论何首乌以生药6 g/kg的剂量长期给药对肾脏有一定的损伤,其损伤程度为醇提法>配方颗粒>水提法。建议临床治疗需谨慎应用何首乌,宜采用水煎剂,需定期检测肾功能(特别是β2-MG),以免对病人引起不可逆的肾损伤。
Objective To investigate the effects of decoction,ethanol extract,and formulated granules of different processed products of Polygonum multiflorum in conventional doses on kidney injury,renal cell apoptosis and expression of related protein in SD rats. Methods Half male and half female SD rats were randomly divided into 7 groups according to their body weight： normal control group,traditional water extract of raw Polygonum multiflorum group（SW group）,traditional water extract of prepared Polygonum multiflorum group（ZW group）,70% alcohol extract of raw Polygonum multiflorum group（SA group）,70% alcohol extract of prepared Polygonum multiflorum group（ZA group）,raw Polygonum multiflorum granules without water decocting extraction group（SK group） and prepared Polygonum multiflorum granules without water decocting extraction group（ZK group）. Among them,the rats in the normal group were intragastrically given distilled water,and the other groups were treated with the corresponding drug liquids [6 g/（kg·d） ofcrude drug] for consecutive 30 days. At the end of the experiment,changes in the levels of blood urea nitrogen（BUN）,creatinine（Crea）,uric acid（UA） and β2-microglobulin（β2-MG） were measured by a semiautomatic biochemical analyzer. In addition,the histopathological changes of kidney tissues were examined,renal cell apoptosis was detected by TUNEL assay,and the expression of Bcl-2 and BAX was determined by immunohistochemical analysis. Results Compared with the normal control group,the levels of BUN in each drug administration group were significantly lower（P〈0. 05,P〈0. 01）. The levels of Crea,UA,and β2-MG in each drug administration group all showed an increasing tendency compared with the normal control group,especially,the level of β2-MG in the ZA group was significantly increased（P〈0. 05）. The result of TUNEL assay showed that the average optical density in each drug administration group was significantly higher than that in the normal control group（
作者
李贺芝
段思明
王茜
刘阳
LI Hezhi;DUAN Siming;WANG Xi;LIU Yang(Hebei Women’s Vocational College,Shijiazhuang 050091,China;Hebei University of Chinese Medicine,Shijiazhuang 050200)
出处
《中国比较医学杂志》
CAS
北大核心
2018年第5期81-87,94,共8页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金青年基金项目(编号:81503268)
河北省高等学校科学技术研究青年拔尖计划项目(编号:BJ2016038)
河北省大学生创新创业计划训练项目(编号:201514432016)
关键词
何首乌
肾损伤
细胞凋亡
Polygonum multiflorum
kidney injury
cell apoptosis
