摘要
本研究建立了水产品中9种拟除虫菊酯类农药残留量的快速检测方法,研究了草鱼、鲫鱼、鲢鱼、罗非鱼和对虾对9种拟除虫菊酯类农药的基质效应,并分析了基质种类、基质浓度和目标分析物浓度对基质效应的影响。结果表明,该方法下9种拟除虫菊酯类农药在5.0~750 ng/m L(七氟菊酯为2.5~750 ng/m L,氯菊酯为10~750 ng/m L)浓度范围内线性关系良好,相关系数在0.9996~0.9999。加标浓度为5.0、50和100μg/kg水平下,平均加标回收率在80.1±2.95%~116±2.45%之间,日内精密度和日间精密度分别为0.272%~10.7%和0.164%~12.8%。5种水产品对9种菊酯类农药均存在不同程度的基质效应,其基质效应在0.906~2.45之间,基质效应随基质浓度增加而增强。此外农药浓度对其基质效应也有较大影响,随农药浓度的增加基质效应逐渐减弱。因此在检测水产品中拟除虫菊酯类农药时应采用基质加标的方法减弱或消除基质效应,确保定量检测结果的准确性。
A rapid method was developed for the detection of 9 pyrethroid pesticides in aquatic products in this study. The matrix effects of Ctenopharyngodon idellus, Carassius auratus, Hypophthalmichthys molitrix, Oreochromis spp and Penaeus orientalis on 9 pyrethroid pesticides were studied, and the effects of matrix types, pesticids concentration and matrix concentration on the matrix effects were analyzed. The results showed that the 9 pyrethroid pesticides had a good linear relationship with the concentration in range of 5.0-750 ng/m L（tefluthrin of 2.5-750 ng/m L, pemethrin of 10-750 ng/m L） with the correlation coefficients of 0.9996-0.9999. The average recoveries of standard addition were 80.1±2.95%-116±2.45 % with the concentration of 5.0, 50 and 100 μg/kg, and the intra-day and inter-day precision were 0.272%-10.7 % and 0.164%-12.8 %, respectively. Different five kinds of aquatic products had different degree of matrix effects on 9 pyrethroids, and the matrix effects were in the range of 0.906-2.45. The matrix effects increased with the increase of matrix concentrations, and the Oreochromis spp, Hypophthalmichthys molitrix and Penaeus orientalis improved the matrix effects. In addition, the concentrations of target analysis had a significant influence on its matrix effect, and the matrix effect decreased with the increase of pesticide concentrations. Therefore, the matrix additive method should be used to reduce or eliminate the matrix effect to ensure the accuracy of quantitative result.
作者
李丽春
刘书贵
尹怡
郑光明
马丽莎
戴晓欣
单奇
赵城
LI Li-chun, LIU Shu-gui, YIN Yi, ZHENG Guang-ming, MA Li-sha, DAI Xiao-xin, SHAN Qi, ZHAO Cheng(Pearl River Fisheries Institute, Chinese Academy of Fishery Sciences; Ministry of Agriculture Laboratory of Quality ＆ Safety Risk Assessment for Aquatic Product （Guangzhou） ;Key Laboratory of Recreational Fisheries Research, Ministry of Agriculture, Guangzhou 510380, China)
出处
《现代食品科技》
EI
CAS
北大核心
2018年第4期270-280,220,共12页
Modern Food Science and Technology
基金
国家青年科学基金项目(31501398)
中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(2017HY-ZC0603)
关键词
水产品
拟除虫菊酯
基质效应
检测方法
aquatic products
pyrethroids
matrix effect
detection method
