摘要
旨在为高粱耐盐种质资源的开发利用及耐盐新种质的创新提供一定的理论依据。选用20份高粱种质作为试验材料,采用150 mmol/L Na Cl溶液进行处理,置于人工气候培养室,测定发芽率、发芽势等相关指标;将20份材料置于25℃恒温培养箱中进行催芽,用150 mmol/L Na Cl溶液浇灌,以等量的浇水为对照,调查并比较幼苗成活率、生长速率及枯萎指数。采用隶属函数进行相关性分析,并进行聚类分析。结果表明,在150 mmol/L盐胁迫下,‘3438’、‘航天育种SP42’、‘09305R’、‘3560R’品种的发芽率高于对照,其他指标均小于对照。20份材料中,在芽期鉴选出耐盐材料4份,苗期筛选出3份;相关性大小顺序为相对芽长>相对根长>相对植株鲜重>发芽势;20份材料分成高度耐盐型、耐盐型、中等耐盐型、盐敏感型和高度盐敏感型品种5类。确定150 mmol/L Na Cl选定为高粱品种萌发期耐盐性鉴定的标准盐浓度,确定发芽率、发芽势、根长、叶长、植株鲜重可用于高粱耐盐性的鉴定指标。
The purpose of this study is to provide a theoretical basis for the development and utilization of salt- tolerant germplasm resources and the innovation of new salt-tolerant germplasm of sorghum. 20 accessions of sorghum were used as materials and treated with 150 mmol/L NaCl in the artificial climate chamber. The germination rate and germination potential were detected. The 20 accessions were placed in 25℃ constant temperature incubator for accelerating germination and irrigated with 150 mmol/L NaCl, the control was irrigated with the same amount of water. The seedling survival rate growth rate and wilting index were investigated and compared. The membership function was used for correlation analysis. The cluster analysis was also adopted in this study. The results showed that under the 150 mmol/L NaCl stress, the germination rate of ＇3438＇, ＇space mutation breeding SP42＇, ＇09305R＇ and ＇3560R＇ was higher than that of the control. And other indexes were lower than that at sprouting stage, 3 salt-resistant of the control. In the 20 materials, accessions were selected at the 4 salt-resistant accessions were selected seedling stage. The order of correlation was as following： relative bud length〉 relative root length〉 relative plant weight〉 germination potential. The 20 accessions of sorghum were clustered into five groups, including high salt-tolerant type, salt-tolerant type, medium salt-tolerant type, salt-sensitive type and high salt-sensitive type. In conclusion, 150 mmol/L NaCl is selected as the standard salt concentration for the identification of salt tolerance of sorghum cultivars. The germination rate, germination potential, root length, leaf length and fresh weight of plant could be used as identification indexes for salt tolerance of sorghum.
作者
范娜
白文斌
彭之东
张建华
史丽娟
李光
江佰阳
曹昌林
Fan Na;Bai Wenbin;Peng Zhidong;Zhang Jianhua;Shi Lijuan;Li Guang;Jiang Baiyang;Cao Changlin(Sorghum Research Institute of Shanxi Academy of Agricultural Sciences, Jinzhong Shanxi 030600;Shanxi Province Key Laboratory of Sorghum Genetic and Germplasm Innovation, Jinzhong Shanxi 030600)
出处
《中国农学通报》
2018年第10期82-87,共6页
Chinese Agricultural Science Bulletin
基金
高粱遗传与种质创新山西省重点实验室项目"粒用高粱耐盐种质资源鉴选研究"(2015Q-03)
国家科技支撑计划项目"优质高粱高效生产技术研究与示范"(2014BAD07B02)
山西省科技攻关项目"高粱深加工关键技术研究-醋糟在土壤改良中的应用研究"(20150311003-1)
关键词
高粱
人工模拟
鉴选
耐盐
综合评价
sorghum
artificial simulation
selection
salt tolerance
comprehensive evaluation
