摘要
本试验以高粱品种吉杂319为材料,研究枯草芽孢杆菌(SN3)处理对盐胁迫下种子萌发、幼苗生长的影响及其渗透调节和氧化应激作用。结果表明:盐胁迫显著抑制高粱种子萌发,且盐浓度越高,抑制作用越强。SN3发酵液浸种显著提高高粱种子发芽率、发芽势、发芽指数和活力指数。土壤中添加150 mmol/L NaCl显著抑制高粱幼苗生长,同时显著降低幼苗根系活力、抗氧化酶活性,提高根系活性氧(ROS)、丙二醛(MDA)和渗透调节物质含量。SN3发酵液和菌悬液灌根均能提高盐胁迫下幼苗株高、茎粗、地上部和地下部干重,并显著提高超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性,显著降低ROS、MDA含量,同时提高根系脯氨酸、可溶性糖含量和K^(+)/Na^(+)比值。种子萌发阶段SN3发酵液浸种效果更佳,幼苗生长阶段SN3菌悬液灌根效果更好。这表明枯草芽孢杆菌(SN3)可通过提高高粱幼苗的抗氧化酶活性、渗透调节物质含量来增强ROS、MDA的清除能力,降低细胞的氧化应激作用,减轻盐胁迫对高粱幼苗的伤害,进而促进幼苗生长,提高抗盐性。
In this study, the effects of Bacillus subtilis(SN3) treatment on seed germination, seedling growth, osmotic regulation and oxidative stress of sorghum variety Jiza 319 under salt stress were studied. The results showed that salt stress significantly inhibited germination of sorghum seeds, and the higher the salt concentration was, the stronger the inhibition effect was. Seed soaking in SN3 fermentation broth significantly improved germination rate, germination potential, germination index and vigor index. Addition of 150 mmol/L NaCl in soil significantly inhibited the seedlings growth, significantly reduced root activity and antioxidant enzyme activity, and increased the contents of reactive oxygen species, malondialdehyde and osmoregulation substances. The root irrigation with SN3 fermentation broth and bacterial suspension inreased the plant height, stem diameter, dry weight of aerial part and underground part of seedlings under salt stress, significantly increase the activities of superoxide dismutase, catalase and peroxidase, significantly reduced the contents of ROS and MDA, and increased the proline and soluble protein contents and K^(+)/Na^(+) ratio in roots. The effect of seed soaking with SN3 fermentation broth was better at seed germination stage, and the effect of irrigating the roots with SN3 bacterial suspension was better at seedling growth stage. The results showed that B. subtilis could enhance scavenging capacity of ROS and MDA, reduce oxidative stress of cells, alleviate damage of salt stress to sorghum seedlings, and then promote the growth of sorghum seedlings and improve salt resistance by increasing antioxidant enzymes activities and osmotic adjustment substances contents of sorghum seedlings.
作者
安明哲
张岩
张妤
徐敬业
王洋
An Mingzhe;Zhang Yan;Zhang Yu;Xu Jingye;Wang Yang(Jilin Academy of Agricultural Sciences,Changchun 130033,China;Institute of Agricultural Economy and Information,Jilin Academy of Agricultural Sciences,Changchun 130033,China;Peanut Research Institute,Jilin Academy of Agricultural Sciences,Gongzhuling 136100,China)
出处
《山东农业科学》
北大核心
2022年第12期81-90,共10页
Shandong Agricultural Sciences
基金
吉林省科技厅重点研发计划项目(20200402108NC)
国家现代农业产业技术体系项目(CARS-06-14.5-A12)
国家自然科学基金面上项目(31671712)
吉林省农业科学院创新工程定向委托项目(CXGC2021DX016)。
