摘要
目的：研究滇黄芩（Scutellaria amoena）地上部分的化学成分。方法：采用90%乙醇加热回流提取滇黄芩地上部分,所得浸膏用水悬浮后用乙酸乙酯和正丁醇萃取分为不同极性部位。继而通过硅胶柱色谱,LH-20羟丙基葡聚糖凝胶（Sephadex LH-20）柱色谱及重结晶等方法分离纯化,根据化合物的理化性质和波谱数据（MS,~1H-NMR,^13C-NMR）鉴定化学结构。结果：从滇黄芩地上部分的90%乙醇提取物中分离得到11个化合物,分别鉴定为黄芩素（1）,黄芩苷（2）,千层纸素A（3）,千层纸素A-7-O-β-D-吡喃葡萄糖醛酸苷（4）,汉黄芩素（5）,去甲汉黄芩素（6）,白杨素（7）,白杨素-7-O-β-D-吡喃葡萄糖醛酸苷（8）,野黄芩苷（9）,5,7-二羟基-6,8-二甲氧基黄酮（10）,β-谷甾醇（11）。结论：以上化合物均为首次从该种植物的地上部分中分离得到,化合物9,10为首次从该种植物中分离得到。
Objective：To study the chemical constituents from the aerial parts of Scutellaria amoena.Method：The aerial parts of S.amoena were extracted by using 90% ethanol heat refluxing method,then suspended in H2O and partitioned with ethyl acetate and n-butanol to obtain respective fractions.The components were isolated and purified by silica gel chromatography,polyamide chromatography,Sephadex LH-20 column,recrystallization and other methods.Their chemical structures were identified on the basis of physicochemical properties and spectral analysis（~1H-NMR,^（13）C-NMR and MS）.Result：Eleven compounds were isolated from 90% ethanol extracts and identified as baicalein（1）,baicalin（2）,oroxylin A（3）,oroxylin A-7-O-β-Dglucurunopyranside（4）,wogonin（5）,norwogonin（6）,chrysin（7）,chrysin-7-O-β-D-glucurunopyranoside（8）,scutellarin（9）,5,7-dihydroxy-6,8-dimethoxyflavone（10）,and β-sitosterol（11）.Conclusion：All compounds were isolated from the aerial parts of S.amoena for the first time.Compounds 9 and 10 were isolated from this plant for the first time.
作者
付胜男
虎春艳
刘海鸥
林玉萍
FU Sheng-nan;HU Chun-yan;LIU Hai-ou;LIN Yu-ping(College of Traditional Chinese Medicine （ TCM）, Yunnan University of TCM, Kunming 650500, Chin)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第10期55-59,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
云南省科学技术厅云南中医学院应用基础研究联合专项[2017FF117(-022),2015FB205(-025)]
关键词
滇黄芩
地上部分
化学成分
清热燥湿
Scutellaria amoena
aerial parts
chemical constituents
clearing heat andexpelling dampness
