摘要
研究本试验中6株不同来源的蛹虫草菌株（150707,150715,150725,150808,52244,51762）的菌丝生长特性及其多糖产量。测定这6株蛹虫草菌丝生长速度、分生孢子产量,利用水提醇沉法提取不同菌株粗多糖。研究发现在不同培养基上不同来源的这6株蛹虫草菌丝生长速度、产分生孢子量、多糖得率不同,在加富PDA培养基上生长速度较快,但是在血平板培养基上孢子产量及多糖得率较优。分析得出150707、52244两个菌株孢子产量和多糖产量相对较高,为优势菌株,血平板培养基为蛹虫草孢子及多糖产量较优培养基。
The aim was to study hyphae growth characteristics and polysaccharide yield of Cordyceps militaris strains（150707, 150715, 150725, 150808, 52244, 51762） from different genetic resources. Hyphae growth rate and conidiospores yield of different Cordyceps militaris strains were measured. Crude polysaccharide from different strains was extracted by water extraction and alcohol deposition method. Hyphae growth rate, conidiospores yield and polysaccharide yield rate of these six strains from different genetic resources were different. The hyphae growth rate was faster on enrichment PDA medium, while the conidiospores yield and polysaccharide yield rate of strains was better on blood plate medium. It can be analysed that the strains 150707 and 52244 were dominant strains because their yield of conidiospores and polysaccharide was superior. Blood plate medium was superior medium for the yield of conidiospores and polysaccharide of Cordyceps militaris strains.
作者
郑庆委
张涛
管俊昌
姚苹苹
慈大正
余子辰
袁静涛
冯翔飞
Zheng Qingwei;Zhang Tao;Guan Junchang;Yao Pingping;Ci Dazheng;Yu Zichen;Yuan Jingtao;Feng Xiangfei(Department of Pathogenic Biology and Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, 233030;Food Quality and Safety Specialty, Bengbu Medical College, Bengbu, 233030)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2018年第4期1526-1530,共5页
Genomics and Applied Biology
基金
国家自然科学基金项目(81070506)
安徽省高校自然科学研究项目一般项目(KJ2015B081by)
安徽省大学生创新训练项目(201610367022
201610367018)
安徽省教育厅重点项目(KJ2016A461
KJ2017A222)共同资助
关键词
种质资源
蛹虫草
分生孢子
多糖
Genetic resource
Cordyceps militaris
Conidiophore
Polysaccharide
Hypha
