摘要
目的探讨血清miR-132及miR-183在胃癌诊断中的价值。方法选取2014年1月-2017年3月海南西部中心医院收治的96例胃癌患者(胃癌组)和45例体检正常者(对照组),采用实时定量PCR法检测两组血清miR-132及miR-183表达水平,应用ROC曲线分析miR-132及miR-183对胃癌的诊断价值。结果胃癌组与对照组的性别(男/女:65/31 vs28/17)、年龄[(62.84±10.36)岁vs(60.46±9.72)岁]差异均无统计学意义(P均>0.05)。胃癌组血清miR-132(0.64±0.08 vs0.12±0.03)及miR-183(5.13±0.34 vs 0.87±0.05)表达水平均明显高于对照组(t=11.834、t=15.627,P<0.01)。ROC曲线分析显示,血清miR-132及miR-183联合诊断胃癌的AUC(95%CI)为[0.958(0.892~0.996)],明显优于miR-132[0.864(0.805~0.926)](Z=7.638,P=0.000)和miR-183[0.904(0.845~0.967)](Z=4.926,P=0.016),其敏感度和特异度为96.2%和87.4%。Pearson相关分析显示,胃癌患者血清miR-132与miR-183呈正相关(r=0.758,P<0.01)。结论血清miR-132及miR-183在胃癌患者中明显上调,有望作为胃癌诊断的新型肿瘤标记物。
Objective To explore the diagnostic value of serum miR-132 and miR-183 in patients with gastric cancer. Methods Real time quantitative PCR was used to detect the expression of miR-132 and miR-183 in 96 cases with gastric cancer（gastric cancer group） and 45 cases of normal controls（control group） in our hospital from January 2014 to March 2017. The diagnostic value of miR-132 and miR-183 for gastric cancer was analyzed by ROC curve. Results There was no statistically significant difference in sex（male/female： 65/31 vs28/17） and age [（62.84±10.36） years vs（60.46±9.72） years] between two groups（all P〉 0.05）. The levels of serum miR-132 and miR-183 in gastric cancer group were significantly higher than those in control group [miR-132：（5.13±0.34） vs（0.87±0.05）, P=0.000;（0.64±0.08） vs（0.12±0.03）, P=0.000]. ROC curve analysis showed that AUC（95% CI） of the combination of serum miR-132 and miR-183 in diagnosis of gastric cancer was [0.958（0.892-0.996）], which was superior to miR-132 [0.864（0.805-0.926）]（Z=7.638, P=0.000） and miR-183 [0.904（0.845-0.967）]（Z=4.926, P=0.016）, and its sensitivity and specificity were 96.2% and 87.4%, respectively. Pearson correlation analysis showed that there was a positive correlation between serum miR-132 and miR-183 in patients with gastric cancer（r=0.758, P=0.000）. Conclusion Serum miR-132 and miR-183 are obviously up-regulated in patients with gastric cancer, and are expected to be a novel tumor biomarker for the diagnosis of gastric cancer.
作者
陈卿奇
李巨元
冯彩团
郑继统
赵高传
CHEN Qingqi;LI Juyuan;FENG Caituan;ZHENG Jitong;ZHAO Gaochuan(Department of Gastroenterology, Hainan West Central Hospital, Danzhou 571799, Hainan Province, China)
出处
《解放军医学院学报》
CAS
2018年第4期299-302,共4页
Academic Journal of Chinese PLA Medical School
基金
海南省医药卫生科研基金项目(14A200012)~~
