摘要
目的：研究血清肿瘤标记物癌胚抗原（CEA）、糖类抗原CA724、热休克蛋白60（HSP60）及恶性肿瘤特异性生长因子（TSGF）联合动态检测在胃癌诊断和监控治疗中的临床应用价值。方法：选取t27例胃癌患者（恶性肿瘤组）、50例胃良性病变患者（良性对照组）和50例健康体检者㈣康对照组）为研究对象，采用电化学发光法及酶联免疫法检测其血清CEA、CA724、HSP60、TSGF水平，统计分析其在三组人群血清中的表达，探讨CEA、CA724、HSP60、TSGF联合动态检测在胃癌诊断和监控治疗中的临床应用价值。结果：①胃癌患者血清CEA、CA724、HSP60、TSGF水平与良性对照组和健康对照组比较明显增高，差异均有统计学意义（均P〈0．01），良性对照组和健康对照组比较差异无统计学意义（P〉0．05）；②胃癌患者血清CEA、CA724、HSP60、TSGF水平与患者不同性别、不同年龄、不同肿瘤发生部位无明显相关性（均P〉0．05）；与肿瘤大小、肿瘤侵润胃壁深度、分化程度、临床分期、伴腹腔积液、区域淋巴结转移、远处转移、复发及治疗后明显相关（均P〈0．05）；肿瘤体积越大、浸润胃壁越深血清标记物水平越高；胃癌中晚期组、低分化组、伴腹腔积液组、转移组、复发组及治疗前血清CEA、CA724、HSP60、TSGF水平较早期组、高分化组、无腹腔积液组、无转移组、无复发组及治疗后明显增高（均P〈0．05）；③CEA、CA724、HSP60、TSGF四项联合检测诊断胃癌的敏感性、准确性分别为95．28％、90．96％，与各单项及部分组合检测比较明显提高，差异有统计学意义（均P〈0．05）。结论：血清肿瘤标记物CEA、CA724、HSP60、TSGF联合检测可以明显提高胃癌诊断的敏感性和准确性，从而减少误漏诊，动态检测对判断肿瘤病变范围、程度、复发转移及疗效评价等具有指导意义。
Objective： This study aims to inuestigate the clinical value of serum tumor markers carcinoembryonic antigen CEA, carbohydrate antigen CA724, heat shock protein 60 （HSP60） and tumor specific growth factor （TSGF） joint dynamic detection in the diagnosis and treatment monitoring of gastric cancer. Methods： 127 gastric cancer patients （malignant group）, 50 benign gastric lesions patients （benign group）and 50 healthy subjects （healthy control group）were evaluated^ECL detection method and enzyme-linked immunosorbent assay were used to detect serum concentration of CEA, CA724, HSP60 and TSGF, retrospective statistical analysis was adopted to analyze the expression of tumor markers CEA, CA724, HSP60 and TSGF in the serum of patients with gastric cancer, and the clinical value of combined dynamic detection was explored in the diagnosis and monitoring of＂ gastric cancer. Results： （~）Serum concentrations of tEA, CA724, HSP60 and TSGF in patients with gastric cancer were significantly higher than that of benign group and the healthy control group, the difference was statistically significant （P〈0.01）. ②There was no significant correlation with patients＇sex, age and the site of tumor of the serum concentrations of CEA, CA724, HSP60 and TSGFin patients with gastric cancer （P〉O.05）, but was significantly correlated with the tumor size, depth of invasion of the stomach, degree of differentiation, clinical stage, ascites, regional lymph node metastasis, distant metastasis, and recurrence after treatment （P〈0.05）. ③The application of CEA, CA724, HSP60 and TSGF four tumor markers combined in the diagnosis of gastric cancer, the sensitivity accuracy improved significantly respectively, 95.28%, 90.96%. There was statistically significant with the individual and every three in combination （P〈0.05）. Gonclusiorls： Serum tumor markers CEA, CA724, HSP60 and TSGF combined dynamic detection improve the diagnostic etTlcacy of gastric cancer, reducing misdiagnosis and missed dia
作者
张荣华
王笑峰
ZHANG Rong-hua;WANG Xiao-feng(Medical department of Qingdao University, Shandong Qingdao 266021)
出处
《医学检验与临床》
2018年第1期5-10,共6页
Medical Laboratory Science and Clinics
关键词
胃癌
肿瘤标记物
诊疗
联合检测
Gastric cancer, Tumor markers
Diagnosis
Combined detection
