摘要
研究测定感冒解毒颗粒中橙皮苷的含量测定方法,为感冒解毒颗粒提高标准提供依据。采用高效液相色谱法,色谱柱为Diamonsil﹙钻石﹚C18﹙5μ,150×4.6 mm﹚,流动相为乙腈-0.5%磷酸﹙21∶79﹚,检测波长为283 nm,流速为1.00 m L/min,进样体积为10μL,柱温为室温。橙皮苷在0.04711632~0.4711632μg/m L浓度范围内呈良好的线性关系,线性回归方程为Y=84.606X+3.1169,R=0.9999(n=10)。平均回收率为104%(n=6),RSD=1.92%(n=6),稳定性RSD为1.90%(n=6),重复性RSD为1.27%(n=6),精密度RSD为0.21%(n=6)。得到结论是本方法操作简便,准确,精密度、重现性、稳定性、耐用性良好,可用于感冒解毒颗粒中橙皮苷的含量测定。
In this study we explored the determination method of hesperidin content in Jiedu Granule for Cold,so as to provide reference for the improvement of the standard of the granule. High performance liquid chromatography was adopted,and the parameters were as follows： chromatographic column was Diamonsil（ diamonds） C18（ 5μ,150× 4. 6 mm）,mobile phase was-0. 5% phosphoric acid（ 21∶ 79）,detection wavelength was 283 nm,flow rate was1. 00 m L/min,sample volume was 10μL,and column temperature was at room temperature. Within the concentration range of 0. 04711632 μg/m L to 0. 4711632 μg/m L,hesperidin content was in good linear relationship,Y =84. 606 X ＋ 3. 1169,R = 0. 9999（ n = 10）. The average recovery rate was 104%（ n = 6）,the RSD was 1. 92%（ n =6）,the RSD of stability was 1. 90%（ n = 6）,the RSD of repeatability was 1. 27%（ n = 6）,and the RSD of precision was 0. 21%（ n = 6）. It was concluded that the method is simple and accurate,with good precision,reproducibility,stability and durability,and can be used for the determination of hesperidin content in Jiedu Granule for Cold.
作者
谭霖
徐秋萍
TAN Lin;XU Qiuping(Qiandongnan Inspection Center of Food and Drug, Kaili 556000, Chin)
出处
《贵州科学》
2018年第2期20-24,共5页
Guizhou Science
