摘要
目的:研究芳姜黄酮及其衍生物对α-葡萄糖苷酶和α-淀粉酶活性的影响。方法:分别以4-硝基苯-β-D-吡喃葡萄糖苷(PNPG)和可溶性淀粉为底物,测定芳姜黄酮及其衍生物对α-葡萄糖苷酶和α-淀粉酶活性的抑制率,分析对α-葡萄糖苷酶的抑制类型。结果:芳姜黄酮及其衍生物能够以剂量依赖性、时间依赖性的方式抑制α-葡萄糖苷酶和α-淀粉酶的活性,且对α-葡萄糖苷酶活性的抑制类型为竞争性抑制;计算结果显示,衍生物PY-2-17、PY-2-33和阿卡波糖的IC50值分别为0.397、0.314和0.57 g/L。结论:芳姜黄酮及其衍生物能够抑制ɑ-葡萄糖苷酶和α-淀粉酶活性,且其衍生物PY-2-17和PY-2-33对α-葡萄糖苷酶活性的抑制作用优于阿卡波糖。
Objective： To determine the effect of ar-turmerone and its derivatives on the activity of α-glucosidase and α-amylase. Methods： Using p-nitrophenyl-β-D-galactopyranoside（ PNPG） and soluble starch solution as substrate respectively to determine the inhibitory rate of ar-turmerone and its derivatives on α-glucosidase and α-amylase,and its inhibitory type on α-glucosidase. Results： Ar-turmerone and its derivatives exhibited inhibition against α-glucosidase and α-amylase with time and dose-dependent feature. Moreover,the inhibitory type was competitive against the activity ofα-glucosidase.Calculation indicated that the derivatives PY-2-17,PY-2-33 and Acasbose IC50 values were0. 397 g/L,0. 314 g/L and 0. 57 g/L respectively. Conclusions： Ar-turmerone and its derivatives demonstrated the inhibitory effect against activity of ɑ-glucosidase and α-amylase,in which the derivatives PY-2-17 and PY-2-33 are superior to Acasbose with α-glucosidase inhibition.
作者
肖露
陈兰
荣冬芸
王叶
曹煜
吴春维
XIAO Lu;CHEN Lan;RONG Dongyun;WANG Ye;CAO Yu;WU Chunwei(Guizhou Medical University, Guiyang 550004, Guizhou, China;Department of Physical Examination, the Ajfiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China)
出处
《贵州医科大学学报》
CAS
2018年第4期400-405,共6页
Journal of Guizhou Medical University
基金
贵州省产业技术创新战略联盟项目[黔科合平台人才(2016)5501号]
贵阳市科技局科技类项目[筑科合同(20161001)号]
