摘要
目的:研究microRNA-151-5p(miR-151-5p)在两肾一夹(2K1C)肾血管性高血压大鼠中的表达,并为miR-151-5p参与调节血管内皮细胞功能提供理论依据。方法:建立2K1C大鼠模型,获得胸主动脉血管内皮,采用荧光定量PCR技术检测血管内皮细胞中miR-151-5p的表达,通过数据库及生物信息学软件预测miR-151-5p的靶基因,并对靶基因进行GO富集和KEGG pathway分析。结果:与假手术组大鼠相比较,实验组大鼠胸主动脉血管内皮细胞miR-151-5p的表达量显著升高(P<0.05)。GO分析显示miR-151-5p的靶基因参与蛋白加工水解、Notch受体加工等多种生物学功能(P<0.01);KEGG pathway分析显示miR-151-5p的靶基因参与Notch信号通路、血管平滑肌收缩、代谢途径、细菌感染和RNA转运等信号通路。结论:2K1C大鼠血管内皮细胞中miR-151-5p表达升高,可能通过对其靶基因APH1A的调控参与血管内皮细胞功能调节。
Objective： To study the expression of microRNA-151-5 p（miR-151-5p） in two kidney one clip（2K1C） renovascular hypertensive rats, and provide a theoretical guidance for miR-151-5p to participate in the regulation of vascular endothelial cell function.Methods： 2K1C rat model was established to obtain the endothelium of thoracic aorta. The expression of miR-151-5p in vascular endothelial cells was detected by fluorescent quantitative PCR. We also predicted target genes of miR-151-5p by database and bioinformatics software. Furthermore, we analyzed the function of target gene by KEGG and GO pathways. Results： Compared with the sham group, the expression of miR-151-5p in thoracic aorta endothelial cells in experimental group was significantly higher（P0.05）. In GO analysis, the target genes of miR-151-5p were enriched in many biological process,such as regulation of protein processing and regulation of Notch receptor processing（P0.01）. In KEGG pathway, the target genes of miR-151-5p were involved in Notch signaling pathway, vascular smooth muscle contraction, metabolic pathway, bacterial infection and RNA transport pathway. Conclusion：MiR-151-5p expression increased in 2K1C rats, it may regulate the function of vascular endothelial cell by its target gene APH1A.
作者
冯月
陈珍珍
胡海
于慧
王占黎
FENG Yue, CHEN Zhen-zhen, HU Har, YU HuY, WANG Zhan-li(1 Graduate School; 2 Department of Pathophysiology; 3 The Inner Mongolia Autonomous Region Key Laboratory of disease-related biomarkers, Institute of Molecular Medicine of Baotou Medical College, The Second Affiliated Hospital of Baotou Medical College, Baotou, Inner Mongolia, 014030, Chin)
出处
《现代生物医学进展》
CAS
2018年第2期239-242,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81460049
81560057)
