摘要
目的：研究当归补血汤对内质网应激特有的Caspase-12凋亡途径的影响,探讨当归补血汤对肾脏的保护机制。方法：60只SD大鼠腹腔内注射链脲佐菌素（STZ）结合高脂高糖饮食建立大鼠糖尿病肾病模型（DN）,喂养8周后,观察大鼠的毛色、精神状态等一般状态;酶偶联比色法检测尿蛋白、BUN、尿肌酐等反应肾功能的相关指标;光镜观察其肾脏病理变化;RT-PCR、western Blot及免疫组化检测GRP78、Caspase-12的蛋白及m RNA表达水平;TUNEL检测肾组织细胞凋亡情况。结果：DN组大鼠空腹血糖、尿蛋白、BUN等明显升高,肾功能受损严重,肾脏组织GRP78、Caspase-12的蛋白及m RNA含量增加,光镜下肾脏结构病变明显,肾组织细胞凋亡率增高;当归补血汤治疗后肾功能损伤减轻,GRP78、Caspase-12的蛋白及m RNA含量降低,光镜下肾组织病变改善,大鼠肾组织凋亡率降低。结论：当归补血汤能抑制内质网应激特有Caspase-12凋亡途径,改善糖尿病肾病大鼠肾功能,减少肾组织过度凋亡。
Objective：To study the effect of Danggui Buxue Decoction on endoplasmic reticulum stressspecific Caspase-12 apoptosis way in diabetic rats and to explore its protection mechanisms. Methods：Sixty SD rats were given STZ ＋ high-fat feed,sacrificed after eight weeks,observed the general condition of these rats,measured blood glucose,and the indicators that related with renal function,enzyme-linked immunosorbent assay to detect the contents of urinary MAU,optical microscope were used to observe the kidney structure change. The total expression of GRP78/Caspase-12 m RNA and protein was determined by real-time PCR,Western blotting and immunohistochemical respectively. The apoptosis of kidney tissues cells was determined by TUNEL assay. Results：The DM group had a significantly higher level of blood glucose,MAU,BUN,serumcreatinine. renal function is seriously damaged;The expression of GRP78 and Caspase-12,both m RNA and protein,were higher;optical microscopically kidney damage was significantly serious;The amount of kidney tissues apoptosis cells significantly were higher. After treatment with Danggui Buxue Decoction treatment,renal function was relieved;The expression of GRP78 and Caspase-12,both m RNA and protein,were dropped;The amount of kidney tissues apoptosis cells were down;optical microscopically kidney damage was significantly relieved. Conclusion：This study proved that Danggui Buxue Decoction can inhibite endoplasmic reticulum stress-specific Caspase-12 apoptosis way,protects the renal function,decline kidney tissues apoptosis in diabetic rats.
作者
张思泉
帅瑜
张莹雯
沈鑫
ZHANG Siquan, SHUAI Yu, ZHANG Yingwen, SHEN Xin(Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei, China)
出处
《辽宁中医药大学学报》
CAS
2018年第3期20-24,共5页
Journal of Liaoning University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81373793)
