摘要
建立一种高效液相色谱法同时检测饮料产品中的5种常见色素的方法。方法采用waters OASIS WAX 3 mg/150 mL固相萃取柱净化,Waters sunfire C-18色谱柱(4.6×250 mm,5μm)进行分离,0.02 mol/mL乙酸铵与乙腈体系洗脱,紫外检测器检测,外标法定量分析。结果表明,5种色素浓度在0.5~50μg/mL范围内线性良好,相关系数均不低于0.999;检测限:苋菜红:0.08μg/g、胭脂红:0.09μg/g、日落黄:0.07μg/g、诱惑红:0.10μg/g、碱性橙21:0.15μg/g;样品加标回收率均在90%~110%之间;精密度和重复性的相对标准偏差(RSD)均在5.0%以内。该方法具有操作简单、准确度高、灵敏度高等优点。
A method for determination of 5 common colorants in beverages by SPE-RP-HPLC method was established. Samples were purified by waters OASIS WAX （3 mg/150 mL） SPE column. The analysis was performed on Waters sunfire C-I 8 column （4.6×250 mm, 5 μm）, 0.02 mol/mL ammonium acetate solution and acetonitrile systern was selected as elution, and UV detector was used to test samples, external standard method for quantitative analysis. The results of 5 common colorants showed good linear correlations in the concentration between 0.5-50 μg/mL; the correlation coefficients were greater than 0.999; Detection limits were as follows： amaranth 0.08 μg/g, carmine 0.09 μg/g, sunset yellow 0.07 μg/g, allure red 0.10/ag/g, Basic Orange 21 0.15 μg/g; The recoveries for samples were： 90 %-110 %; the precision and repeatability were≤ 5.0 % （n=6）, The method was proved to be simple, accurate and sensitive.
作者
陈松润
何权
黄娟
Chen Songrun 1,2,He Quan 1,2, Huang Juan1,2(1. China Foods Detection and Research Institute Co., Ltd. Dongguan 523000;2. China National Adolescent Food Quality Supervision and Inspection Center, Dongguan 523000, Chin)
出处
《广东化工》
CAS
2018年第6期227-228,共2页
Guangdong Chemical Industry
