摘要
检测癌胚抗原相关黏附分子1(CEACAM1)和基金属蛋白酶-2(MMP-2)在AGS和SGC-7901胃癌细胞系中的表达,探讨在胃癌细胞发生发展和侵袭转移中的作用。采用实时定量PCR检测两种细胞株中CEACAM1和MMP-2的mRNA表达;采用Western blot检测两种细胞株中CEACAM1和MMP-2的蛋白相对表达量;采用Trasnwell小室分析两种细胞株的侵袭能力。AGS的CEACAM1和MMP-2的mRNA及蛋白表达量均高于SGC-7901,差异有统计学意义(P<0.05)。AGS的穿膜细胞数为(147.2±3.6),高于SGC-7901的穿膜细胞数(83.5±3.9),差异有统计学意义(P<0.05)。CEACAM1和MMP-2的表达与胃癌细胞分化程度相关,且表达程度越高,其侵袭、转移能力越强。
To investigate the expression of CEACAM1 and MMP-2 and to explore the effects of CEACAM1 and MMP-2 on invasion and metastasis of gastric cancer cell lines AGS and SGC-7901,RT-PCR was used to detect the expressions of mRNA in CEACAM1 and MMP-2. Western blot was used to measure the relative expression of proteins in CEACAM1 and MMP-2. Trans well chambers were used to investigate the migration of the two cell lines. The mRNA level and relative protein content in AGS were higher than those in SGC-7901（P〈0. 05）. For AGS,the count of transmembrane cell was more than SGC-7901（147. 2 ± 3. 6 vs. 83. 5 ± 3. 9,P〈0. 05）. The expression of CEACAM1 and MMP-2 was connected with the differentiation degree of gastric cancer cell,and higher levels may promote the invasion and metastasis of cancer cell.
作者
向培
陈艳
潘棋
黄逸姣
谢楠岚
朱为民
XIANG Pei, CHEN Yan, PAN Qi, HUANG Yi-jiao, XIE Nan-lan, ZHU Wei-min(Department of Integrated TCM and Western Medicine, Wuxi No. 2 Hospital of TCM, Wuxi 214000, Chin)
出处
《药物生物技术》
CAS
2018年第1期31-33,共3页
Pharmaceutical Biotechnology
关键词
胃癌
癌胚抗原相关黏附分子1
基金属蛋白酶-2
分化程度
侵袭
转移
Gastric cancer, CEACAM1, Matrix metalloproteinase-2 （ MMP-2 ）, Differentiation degree, Invasion, Metastasis
