摘要
针对质谱在磷酸化肽分析过程中检测灵敏度低的问题,本文在移液器吸头尖端原位制备了氨基功能化整体材料,并将其应用于磷酸化肽的快速富集.基于该材料所建立的磷酸化肽富集方法操作简便、分析时间短,并且能够有效避免富集过程中材料与溶液分离不完全所导致的样品损失等问题.而在不同组成的上样溶液条件下,氨基功能化整体材料对单磷酸化肽和多磷酸化肽表现出了不同的磷酸化肽富集选择性,因而借助于对上样溶液组成的调控,该材料可以分别实现对磷酸化肽的全富集以及对多磷酸化肽的选择性富集.
To overcome the concern pertaining to low sensitivity in phosphopeptide analysis by mass spectrometry, amino-functionalized monolithic tips were designed for rapid phosphopeptide enrichment. Phosphopeptide enrichment using amino-functionalized monolithic tips featured ease of operation and low time consumption and helped preventing sample loss caused by incomplete separation of material and solution. In solutions with different compositions, the synthesized monolithic tips showed different enrichment selectivity toward mono- and multiphosphopeptides. Both global enrichment of all phosphopeptides and selective enrichment of multiphosphopeptides were realized by modulating the composition of solution for sample loading.
出处
《中国科学：生命科学》
CSCD
北大核心
2018年第2期207-214,共8页
Scientia Sinica(Vitae)
基金
国家自然科学基金(批准号：21575007,21527809,21322505)资助
关键词
氨基功能化整体材料
磷酸化肽
快速富集
可控富集选择性
amino-functionalized monolithic tip, phosphopeptide, rapid enrichment, tunable selectivity
