摘要
蛋白质O-GalNAc糖基化作为蛋白质翻译后修饰的一种重要形式,参与重要的生理和病理过程.在血浆及其他分泌体系中,O-GalNAc修饰的异常改变可能作为疾病预警的标志分子.鉴于O-GalNAc结构的多样性,系统性分析O-GalNAc修饰具有极大的挑战.随着富集方法、质谱分析技术以及数据解析工具的发展,近年来O-GalNAc修饰的研究取得了一系列进展,促进了对蛋白O-GalNAc糖基化在人类健康中所起作用的深入理解.本文主要介绍近年来分泌体系中的O-糖基化蛋白、O-糖链以及O-糖基化位点的富集与鉴定方法和最新进展.
O-GalNAc is one of the most important post-translational modifications of protein, which regulates key physiological and pathological processes. In plasma and other secretion system, aberrant O-glycosylation may serve as early warning sign for diseases. Because of the diversity of O-linked glycan structures, systemic analysis of O-GalNAc poses a great challenge. With the development of enrichment methods, mass spectrometry technologies and data analysis tools, a series of progress has been reported in the field of O-glycosylation research, which extends our understanding of the function of the O-glycoprotein and O-glycoproteome in human health. In this paper, we try to review main progress of the techniques and their applications in O-glycoprotein and O-glycoproteome studies in secretory systems.
出处
《中国科学：生命科学》
CSCD
北大核心
2018年第2期124-133,共10页
Scientia Sinica(Vitae)
基金
国家重点研发计划(批准号：2017YFF0205400,2016YFA0501300)
国家自然科学基金(批准号：81530021)资助
