摘要
目的建立HPLC法测定瓶尔小草中槲皮素含量的方法。方法采用Kromasil 100-5-C18色谱柱(250mm×4.6mm,5μm),流动相为乙腈(A)-0.2%醋酸水(B)(27∶73)等度洗脱,检测波长为360nm,流速为1.0m L·min-1,柱温:30℃。结果槲皮素进样量在26.78~178.56ng范围内与峰面积呈良好线性关系(r=0.9997,n=6),槲皮素的平均回收率为100.31%(RSD=1.204%)。结论该法简便、准确、可靠,可用于瓶尔小草中槲皮素的含量测定。运用该法测定不同干燥方法的瓶尔小草样品中槲皮素含量,结果二者槲皮素含量差距较大,低温烘干样品中槲皮素含量明显高于阴干样品。
OBJECTIVE To establish a method for the quantification of quercetin in Ophioglossum vulgatum L. by HPLC. METHODS The experimental conditions list as follow Kromasil 100-5-C18（250 mm × 4. 6 mm,5μm）column,mobile phase of acetonitrile-acetic acid water（27：73,p H = 2 ~ 3）,30℃,flow rate of 1. 0 m L·min^-1,detection wavelength of 360 nm. RESULTS The method had good linear relationship within the range of 26. 78 ~178. 56 ng（ r = 0. 9997） for quercetin. The average recoveries（ n = 6） were 100. 31%（ RSD = 1. 204%） for quercetin. The contents of quercetin in Ophioglossum vulgatum L. by dring in the shade. and dring in low temperature were0. 0194% and 0. 0404% respectively. CONCLUSION The content of quercetin in Ophioglossum vulgatum L. by different drying methods. was different. This method is convenient,accurate and reliable for the quantification of quercetin in Ophioglossum vulgatum L.
出处
《海峡药学》
2018年第2期73-75,共3页
Strait Pharmaceutical Journal
基金
川北医学院2015年校级科研发展计划项目(项目编号:CBY15-A-YB26)
