摘要
植物在受到病原菌侵染时,ERF转录因子被诱导调控抗病相关基因的表达。为了研究棉花AP2/ERF-B3类转录因子在棉花抗病调控中的作用,用电子克隆及RT-PCR技术,从陆地棉抗枯萎品种‘中棉所12’根部c DNA克隆得到一个新的ERF转录因子Gh ERF5-3(登录号:MF197875)。利用q RT-PCR检测枯萎病及不同激素处理后该基因的表达。结果表明:其c DNA全长为672 bp,编码223个氨基酸,分子量24.95 k D,等电点为9.04,含有一个典型的保守AP2结构域,通过进化树分析属于B3亚组。枯萎病菌侵染后,随着侵染时间的延长,Gh ERF5-3基因呈现出先增后降的趋势,在24 h时间点,其相对表达量达到峰值;ET和Me JA处理后,Gh ERF5-3基因呈上调表达。SA处理后为下调表达。推断Gh ERF5-3基因响应枯萎病菌。
When plants are infected by pathogens, ERF transcription factors are induced to regulate the expression of disease resistance related genes. To investigate the role of cotton AP2/ERF-B3 transcription factors in cotton disease resistance regulation, a new ERF transcription factor named GhERF5-3 （accession number： MF197875） was cloned from the root cDNA of the ＂Zhong mian suo 12＂ by electronic cloning and RT-PCR technology. qRT-PCR was conducted to detect the expression of this gene after treatment of blight and different hormones. Results showed that the gene cDNA was of 672 bp, encoding 223 amino acids, with the molecular weight of 24.95 kD, and the isoelectric point of 9.04. It contained a typical conservative AP2 domain. Phylogenetic tree analysis showed that GhERF5-3 belonged to ERFB3 subgroup. After the infection of blight, with the extension of infection time, the GhERF5-3 gene showed the trend of first increasing and then decreasing, and the relative expression reached its peak at 24 h. After being treated by ET and MeJA, the expression level of GhERF5-3 gene increased. While its expression decreased after the SA treatment. Therefore, we speculated that GhERF5-3 genes might participate in the defense responses to blight.
出处
《分子植物育种》
CAS
CSCD
北大核心
2018年第4期1050-1056,共7页
Molecular Plant Breeding
基金
国家自然基金项目(31260358)资助
