摘要
大丽轮枝菌是影响棉花生长发育的一种通过土传真菌维管束的病菌,严重影响棉花纤维的产量和品质。为研究棉花GDSL脂肪酶(GDSL lipase,GLIP)在植物响应大丽轮枝菌中的重要功能,本研究利用大丽轮枝菌悬浮液对新陆早42号及其转GhGLIP基因的棉花株系叶片进行处理,分析转GhGLIP棉花株系对大丽轮枝菌的生理生化响应。结果表明,棉花GDSL脂肪酶基因GhGLIP能够响应大丽轮枝菌的刺激,转GhGLIP基因棉花株系可显著抑制大丽轮枝菌导致的叶片细胞死亡。与未处理相比,经大丽轮枝菌处理后的野生型和转基因棉花株系植株的GDSL脂肪酶活力显著增加。同时,转基因棉花株系植株过氧化氢酶、过氧化物酶、超氧化物歧化酶、苯丙氨酸解氨酶和多酚氧化物酶的表达显著提升,说明GhGLIP基因诱导了棉花对大丽轮枝菌的抗性。本研究为棉花抵抗大丽轮枝菌的分子机制解析,及进一步利用基因工程技术培育抗病棉花材料提供了有效参考。
Verticillium dahilae is a soil-brone fungal pathogen through vasculature, and affects the yield and quality of cotton fiber seriously. To investigate the important function of cotton GDSL lipase(GLIP) in response to Verticillium dahilae,physiological analyses of wild-type(WT) cotton(Xinluzao 42) and transgenic cotton overexpressing GhGLIP under Verticillium dahilae treatment were performed in this study. The results indicated that the expression of GhGLIP gene was induced under Verticillium dahilae stimulation, and transgenic cotton plants showed obviously inhibition of leaf cell death that was caused by Verticillium dahliae. Compared with the untreated control, GDSL lipase enzyme activity of both WT and transgenic cotton plants increased significantly after Verticillium dahilae treatment. Meanwhile, in transgenic cotton plants, the enzyme activities of catalase(CAT), superoxide dismutase(SOD), peroxidase(POD), phenylalanine ammonialyas(PAL) and polyphenoloxidase(PPO)were significantly enhanced, demonstrating that GhGLIP induced the resistance of cotton plants to Verticillium dahilae. These results provided an effective reference for elucidating the molecular mechanism of cotton resistance to Verticillium dahliae and improvement of cotton breeding through genetic engineering technology.
出处
《石河子大学学报（自然科学版）》
CAS
2017年第4期420-424,共5页
Journal of Shihezi University(Natural Science)
基金
国家自然科学基金项目(31660408)
新疆兵团现代农业科技攻关与成果转化项目(2016AC017)
