摘要
目的探讨一氧化氮合酶(i NOS)基因多态性与冠心病的关联性。方法病例组为291例冠心病患者,年龄、性别匹配的健康体检者487例为对照组。采用Taqman探针荧光定量PCR技术检测rs2779248 C/T、rs1137933 C/T的基因型分布情况。结果经校正传统危险因素后,携带rs2779248 TT基因型的个体患冠心病的风险比携带CC基因型的高2.58倍(95%CI:1.01~6.67);而携带rs1137933 CT+TT基因型的个体患冠心病的风险比携带CC基因型的高1.36倍(95%CI:1.00~1.85),突变T等位基因可能增加冠心病发病风险(OR=1.36,95%CI:1.04~1.77)。各基因型间空腹血糖水平存在明显差异。结论 i NOS基因多态性与中国汉族人群冠心病发病风险相关,i NOS基因可能通过调节血糖影响冠状动脉粥样硬化斑块形成,进而增加冠心病的发病风险。
Objective To explore the association between inducible nitric oxide synthase（iNOS） gene polymorphisms and coronary heart disease （ CHD） in Chinese Han population. Methods A case- control study was conformed, inc1uded 291 CHD patients and 487 healthy subjects of the age and sex-matched in Chinese Han population. The genotype of rs2779248 （ C/T） and rs1137933 （ C/T） were determined by Taqman SNP genotyping assays. Results After adjusted by conventional risk factors,we found that individuals with rs2779248 TT genotype may have higher risk of CHD（ OR =2. 58,95% CI：1. 01-6. 67）than individuals with CC genotype：while individuals with CT ＋ TT genotype also have higher risk of CHD than those with CC genotype,the odds ratio was 1. 36 （95% CI： 1. 00-1. 85 ） . Individuals with rs2779248 TT genotype had significant higher level of fasting blood glucose than those with rs 2779248 CC genotype. Conclusions 0ur study reveals the polymorphisms of iN0S gene is associated with CHD in Chinese Han population, iN0S gene may increase the incidence of CHD by affecting the formation of coronary atherosclerotic plaque by regulating blood glucose.
出处
《中国心血管杂志》
2018年第1期31-35,共5页
Chinese Journal of Cardiovascular Medicine
基金
国家自然科学基金项目(814202755)
广东省科学技术厅公益研究和能力建设专项(2014A020212380)
深圳市福田区卫生公益性科研项目(FTWS20160030)~~
关键词
一氧化氮合酶
冠状动脉疾病
多态性
单核苷酸
危险因素
Nitric oxide synthase
Coronary artery disease
Polymorphism, single nucleotide
Risk factors
