摘要
目的探讨诱导型一氧化氮合酶（inducible nitric oxide synthase, iNOS）基因多态性与中国汉族人群缺血性卒中的相关性。
方法纳入首发缺血性卒中的患者和年龄、性别相匹配的健康体检对照者，采用Taqman探针荧光定量聚合酶链反应技术检测rs2779248 C/T和rs1137933 C/T多态性的基因型分布。
结果共纳入246例缺血性卒中患者和246名对照者。rs2779248 CC、CT和CC基因型在病例组的分布频率分别为57.7%、36.6%和5.7%，对照组为68.7%、28.0%和3.3%，病例组T等位基因频率显著高于对照组（24.0%对17.3%；P＝0.015）；多变量logistic回归分析显示，在校正年龄、性别、高血压、糖尿病等危险因素后，CT＋TT基因型携带者缺血性卒中风险是CC基因型携带者的1.64倍（优势比1.64，95%可信区间1.07～2.51；P＝0.022）。rs1137933 CC、CT和TT基因型在病例组的分布频率分别为58.1%、37.8%和4.1%，对照组为68.3%、29.3%和2.4%，病例组T等位基因频率显著高于对照组（23.0%对17.1%；P＝0.013）；多变量logistic回归分析显示，在校正传统危险因素后，TT＋CT基因型携带的缺血性卒中风险是CC基因型携带者的1.60倍（优势比1.60，95%可信区间1.05～2.46；P＝0.030）。
结论iNOS基因rs2779248 C/T和rs1137933 C/T多态性与中国汉族人群缺血性卒中的发病风险有关。
ObjectiveTo investigate the correlation between inducible nitric oxide synthase （iNOS） gene polymorphism and ischemic stroke in Chinese Han population.
MethodsPatients with first-ever stroke and the age-and sex-matched healthy controls were enrolled in the study. Taqman probe fluorescence quantitative polymerase chain reaction technique was used to detect the genotype distribution of rs2779248 C/T and rs1137933 C/T polymorphisms.
ResultsA total of 246 patients with ischemic stroke and 246 controls were enrolled. The distribution frequencies of rs2779248 CC, CT and TT genotypes in the patient group were 57.7%, 36.6%, and 5.7%, respectively, and in the control group were 68.7%, 28.0%, and 3.3%, respectively. The T allele frequency of the patient group was significantly higher than that of the control group （24.0% vs. 17.3%; P=0.015）. Multivariate logistic regression analysis showed that after adjusting for the risk factors including age, sex, hypertension, and diabetes, the risk of ischemic stroke in the CT＋ TT genotype carriers was 1.64 times of the CC genotype carriers （odds ratio 1.64, 95% confidence interval 1.07-2.51; P=0.022）. The distribution frequencies of rs1137933 CC, CT and TT in the patient group were 58.1%, 37.8%, and 4.1%, respectively and in the control group were 68.3%, 29.3%, and 2.4%, respectively. The T allele frequency of the patient group was significantly higher than that of the control group （23.0% vs. 17.1%; P=0.013）. Multivariate logistic regression analysis showed that after adjusting for traditional risk factors, the risk of ischemic stroke of patients with the TT＋ CT genotype carriers was 1.60 times of the CC genotype carriers （odds ratio 1.60, 95% confidence interval 1.05-2.46; P=0.030）.
ConclusionsThe rs2779248 C/T and rs1137933 C/T polymorphisms in iNOS gene may be associated with the risk of onset of ischemic stroke in Chinese Han population.
出处
《国际脑血管病杂志》
2015年第10期756-760,共5页
International Journal of Cerebrovascular Diseases
基金
国家自然科学基金（18114202755）
广东省科学技术厅公益研究和能力建设专项（2014A020212380）
