摘要
目的:建立爱迪胶囊B的质量标准。方法:采用TLC法对主要成分黄芪、何首乌、天麻进行定性鉴别;采用HPLC法测定天麻中有效成分天麻素的含量,色谱柱为Eclipse Plus C_(18)柱(250 mm×4.6 mm,5μm),以乙腈-0.05%磷酸溶液(2∶98)为流动相,检测波长为220 nm,流速1.0 ml·min^(-1),柱温25℃。结果:黄芪、何首乌和天麻TLC图谱斑点清晰,分离度好,阴性样品无干扰;天麻中有效成分天麻素线性浓度为8.532~208.8μg·ml^(-1)(r=1.000 0),平均回收率为100.30%,RSD为0.37%(n=6)。结论:本方法操作简单,重复性好,可用于爱迪胶囊B的质量控制。
Objective： To establish the quality standard for Aidi B capsules. Methods： Astragalus membranaceus,Fallopia multiflora and Gastrodiae elata were identified by TLC qualitatively. The content of gastrodin was determined by HPLC. The chromatographic separation was carried out on an Eclipse Plus C_（18） column（ 250 mm × 4. 6 mm,5 μm）. The mobile phase consisted of acetonitrile-0. 05% phosphoric acid solution（ 2∶ 98） at a flow rate of 1. 0 ml·min^（-1）. The detection wavelength and the column temperature was220 nm and 25℃,respectively. Results： The spots in TLC were clear without any interference. The linear range of gastrodin was 8. 532-208. 8 μg·ml^（-1）（ r = 1. 000 0）,and the average recovery was 100. 30%（ RSD = 0. 37%,n = 6）. Conclusion： The method is simple with good repeatability,which can be used for the quality control of Aidi B capsules.
出处
《中国药师》
CAS
2018年第2期351-353,共3页
China Pharmacist
