摘要
目的:研究牛膝的化学成分。方法:将牛膝根切成小段,用95%乙醇提取,将浓缩的提取液经过Sephadex LH-20、ODS、硅胶柱层析等多种柱色谱技术,以及制备液相色谱技术进行分离纯化,根据所得化合物的理化性质和波谱数据进行结构鉴定。结果:从牛膝的95%乙醇提物中分离并鉴定13个化合物,分别为:β-蜕皮甾酮(1)、25-R-牛膝甾酮(2)、25-S-牛膝甾酮(3)、水龙骨甾酮(4)、土克甾酮(5)、palythoalone B(6)、尿嘧啶苷(7)、甲基焦谷氨酸(8)、腺嘌呤(9)、掌叶半夏碱丁(10)、N-反式阿魏酰基酪胺(11)、N-顺式阿魏酰基酪胺(12)、N-反式阿魏酰-3-甲氧基酪胺-4'-O-β-D-葡萄糖(13)。结论:其中,化合物5~10系首次从牛膝中分离得到。
Objective： To study the chemical constitutes from the Achyranthis Bidentatae Radix. Methods： The Achyranthis Bidentatae Radix were cut into pieces,and then extracted with 95% ethanol. The concentrated extracting solution was separated and purified by semipreparative HPLC,Sephadex LH-20,and silica gel column chromatography. Their structures were elucidated on the basis of spectral data and physiochemical properties. Results： Thirteen compounds were isolated. They were identified as β-ecdysterone（ 1）,25-R-inokosterone（ 2）,25-S-inokosterone（ 3）,polypodine B（ 4）,turkesterone（ 5）,palythoalone B（ 6）,uracil glucoside（ 7）,methyl pyroglutamic acid（ 8）,adenine（ 9）,pedatisectine D（ 10）,N-trans-feruloyltyramine（ 11）,N-cis-feruloyltyramine（ 12）,N-trans-feruloyl-3-methoxytyramine-4＇-O-β-D-glucopyranoside（ 13）. Conclusion： Compounds 5 ~ 10 are isolated from this plant for the first time.
出处
《中药材》
CAS
北大核心
2017年第8期1842-1845,共4页
Journal of Chinese Medicinal Materials
基金
河南中医药大学科技创新团队
科技创新人才计划资助项目(2015XCXTD02)
河南中医学院省属高校基本科研业务专项支持计划(2014KYYWF-ZZCXZ-01)
