摘要
目的观察夹脊电针对脊髓损伤小鼠模型运动功能及凋亡蛋白的影响。方法 96只C57BL/6雌性小鼠随机分为假手术组、模型组、针刺组和电针组[疏密波(2/100Hz),0.2mA,15min],每组24只,采用腹腔注射麻醉剂制备T10SCI模型。选择T8和T12两对夹脊穴进行针刺治疗,每天1次,干预28d。观察CatWalk步态分析,采用Western Blot测定脊髓P53、Bax、Bcl-2蛋白表达。结果与模型组同期比较,电针组14、28d小鼠爪印面积、支撑时相比增加,P53表达降低,28d下肢摆动速度加快(P<0.05);针刺组28d小鼠下肢摆动速度、支撑时相比增加,7、14、28dP53表达降低(P<0.05);电针组和针刺组3~28dBax表达降低,7~28dBcl-2表达升高(P<0.01,P<0.05)。与针刺组同期比较,电针组28d爪印面积变大、支撑时相比增加,P53表达降低,7、14dBax表达降低,7、28dBcl-2表达升高(P<0.05)。结论夹脊电针可促进脊髓损伤小鼠运动功能恢复,抑制P53、Bax和促进Bcl-2表达。
Objective To observe the effects of electroacupuncture （EA） at Jiaji points（EX-B2） on the expression of apoptosis proteins and the motor function in spinal cord injury （SCI） mice. Methods Ninety-six female C57BL/6 mice were randomly divided into Sham group, model group, acupuncture （AP） group and EA group（dense-disperse wave of 2/100 Hz frequency, 0.2 mA, 15 min）, 24 in each group. SCI model was prepared by intraperitoneal injection of anesthetics. Jiaji points （EX-B2） at T8 and T12 were se- lected. EA and AP were performed every day and continued for 28 days. CatWalk gait analysis were detec- ted. The expression of P53, Bax and Bcl-2 were determined by Western Blot. Results Compared with the model group, print area and duty cycle were enhanced in the EA group at day 14 and day 28, the expres- sion of P53 were decreased （P 〈0.05） ~ the swing speed was increased at day 28 （P 〈0.05）. The swing speed and duty cycle were enhanced in the AP group at day 28 （P 〈0.05）, the expression of P53 were decreased at day 7-28 （P 〈0.05） ; the expression of Bax in the EA and AP group were decreased at day 3-28, the expression of Bcl-2 were increased at day 7-28 （P 〈0.05）. Compared with the AP group, print area and duty cycle were enhanced in the EA group at day 28, the expression of P53 were decreased （P 〈0.05） ~ the expression of Bax in the EA group were decreased at day 7 and 14, the expression of Bcl- 2 were increased at day 7 and 28 （P 〈0.05） . Conclusion EA at jiaji points （EX-B2） could promote the re- covery of locomotor function, inhibit the expression of Bax and P53, and promote the expression of Bcl-2.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2018年第2期219-222,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金青年科学基金项目(No.81503639)
黑龙江省自然科学基金面上项目(No.H2016070)
关键词
脊髓损伤
电针
夹脊穴
运动功能
凋亡蛋白
spinal cord injury
electroacupuncture
Jiaji points （EX-B2）
motor function
apopto- sis proteins
