摘要
目的:本研究分析溶酶体相关4次跨膜蛋白B(lysosome-associated transmembrane protein 4β,LAPTM4B)-35和基质金属蛋白酶(matrix metalloproteinase,MMP)-9在胃癌中的表达及其与临床病理因素和预后的关系。方法:采用q RT-PCR法检测胃癌细胞株及正常胃黏膜细胞株中LAPTM4B-35和MMP-9 m RNA的表达,免疫组织化学分析胃癌石蜡标本LAPTM4B-35和MMP-9蛋白的表达,运用相关手段分析蛋白表达与临床相关性,Log-rank单因素分析和Cox多因素分析对预后进行研究。结果:LAPTM4B-35和MMP-9 m RNA在HGC-27、SGC7901和MGC803胃癌细胞株相对表达量明显高于GES-1胃黏膜细胞株;免疫组织化学结果显示LAPTM4B-35和MMP-9均在细胞质中表达,LAPTM4B-35阳性表达率为50.7%,MMP-9阳性表达率为36.8%;LAPTM4B-35的表达与MMP-9的表达呈正相关;相关分析显示Lauren's分型和肿瘤浸润T分期与LAPTM4B-35表达相关,肿瘤大小和淋巴结转移N分期与MMP-9表达密切相关,预后生存Cox多因素分析结果显示分化程度、淋巴结转移N分期、LAPTM4B-35表达与MMP-9表达是胃癌患者独立预后因素。结论:LAPTM4B-35和MMP-9在胃癌的侵袭和迁移中起着重要作用,其表达为胃癌预后独立因素,可作为胃癌预后指标。
Objective： To investigate the protein expression level of lysosome-associated transmembrane protein 4B-35 （LAPTM4B-35） and matrix metalloproteinase-9 （MMP-9） in gastric cancer （GC） and to determine the relationship of LAPTM 48-35 or MMP-9 expression with clinicopathological characteristics and prognosis of patients with GC. Methods： The messenger RNA （mRNA） expression level of LAPTM4B-35 and MMP-9 was detected by real-time quantitative polymerase chain reaction, while the protein expression level of LAPTM 4B-35 and MMP-9 in GC tissues was analyzed by immunohistochemistry in formalin-fixed samples from 152 patients with GC, and data analysis was performed using statistical methods. Results： The mRNA expression level of both LAPTM4B-35 and MMP-9 in HGC-27, SGC 7901, and MGC803 GC cell lines was higher than that in the GES-1 normal gastric mucosa cell line. LAPTM 4B-35 and MMP-9 proteins mainly localized in the cytoplasm of GC cells. The positive LAPTM4B-35 expression ratio in GC tissues was 50.7%, while the MMP-9 positive ratio was 36.8%. LAPTM4B-35 and MMP-9 were positively related to gastric cancer. Lauren＇s classification and T stage were identified as the relative factors of LAPTM4B-35 expression, while tumor size and N stage were identified as the rela-tive factors of MMP-9 expression in GC tissues. Cox analysis indicated that the degree of differentiation, N stage, LAPTM 4B-35 expression, and MMP-9 expression were independent prognostic indicators for patients with GC. Conclusion： LAPTM4B-35 and MMP-9 play important roles in the aggressiveness of GC, and LAPTM4B-35 and MMP-9 may be promising indicators for the prognosis of patients with GC.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2017年第24期1226-1231,共6页
Chinese Journal of Clinical Oncology
